Literature DB >> 19478443

Crystallographic characterization of the passenger domain of the Bordetella autotransporter BrkA.

Li Zhao1, Nham T Nguyen, Rachel C Fernandez, Michael E P Murphy.   

Abstract

Autotransporters (ATs) are proteins that deliver effectors (the passenger domain) to the surface of Gram-negative bacteria by the type V secretion pathway. The passenger domain of BrkA, a Bordetella pertussis autotransporter mediating serum resistance and adherence, was cloned in a pET expression system and overexpressed in Escherichia coli. The gene product was correctly refolded, purified to homogeneity and crystallized. The crystals diffracted to 2.8 A resolution. The space group was assumed to be P4(1)2(1)2, with unit-cell parameters a = b = 108.19, c = 115.35 A.

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Year:  2009        PMID： 19478443      PMCID： PMC2688422          DOI： 10.1107/S174430910901642X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

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Authors:  R J Read
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2001-09-21

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3.  Identification of secretion determinants of the Bordetella pertussis BrkA autotransporter.

Authors:  David C Oliver; George Huang; Rachel C Fernandez
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

Review 4.  Type V protein secretion pathway: the autotransporter story.

Authors:  Ian R Henderson; Fernando Navarro-Garcia; Mickaël Desvaux; Rachel C Fernandez; Dlawer Ala'Aldeen
Journal:  Microbiol Mol Biol Rev       Date:  2004-12       Impact factor: 11.056

5.  The turn of the screw: variations of the abundant beta-solenoid motif in passenger domains of Type V secretory proteins.

Authors:  Andrey V Kajava; Alasdair C Steven
Journal:  J Struct Biol       Date:  2006-05-04       Impact factor: 2.867

6.  Pertactin beta-helix folding mechanism suggests common themes for the secretion and folding of autotransporter proteins.

Authors:  Mirco Junker; Christopher C Schuster; Andrew V McDonnell; Kelli A Sorg; Mary C Finn; Bonnie Berger; Patricia L Clark
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-20       Impact factor: 11.205

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Structure of Bordetella pertussis virulence factor P.69 pertactin.

Authors:  P Emsley; I G Charles; N F Fairweather; N W Isaacs
Journal:  Nature       Date:  1996-05-02       Impact factor: 49.962

9.  Cloning and sequencing of a Bordetella pertussis serum resistance locus.

Authors:  R C Fernandez; A A Weiss
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441

10.  A conserved region within the Bordetella pertussis autotransporter BrkA is necessary for folding of its passenger domain.

Authors:  David C Oliver; George Huang; Elena Nodel; Steve Pleasance; Rachel C Fernandez
Journal:  Mol Microbiol       Date:  2003-03       Impact factor: 3.501
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1.  A generalised module for the selective extracellular accumulation of recombinant proteins.

Authors:  Yanina R Sevastsyanovich; Denisse L Leyton; Timothy J Wells; Catherine A Wardius; Karina Tveen-Jensen; Faye C Morris; Timothy J Knowles; Adam F Cunningham; Jeffrey A Cole; Ian R Henderson
Journal:  Microb Cell Fact       Date:  2012-05-28       Impact factor: 5.328
  1 in total

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