Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1).

The interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1) triggers the release of large amounts of interleukin-4 from human blood basophils, thus presumably playing an immunomodulatory role during schistosome infection. IPSE/alpha-1 was crystallized and a native X-ray data set was collected to 1.66 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belonged to space group P6(1) or P6(5), with one molecule per asymmetric unit.