BACKGROUND: An improved light activated disinfection technique utilizing a specific photosensitizer formulation, liquid optical-conduit, oxygen-carrier and light energy of appropriate wavelength has been introduced recently. This study tested the efficacy of this improved light activated disinfection on ex vivo biofilms of Enterococcus faecalis at two different stages of maturation. METHODS: Eighty-five tooth sections were prepared and endodontic biofilm of E. faecalis were grown within the root canal. In stage 1, conventional light activated disinfection (LAD), chemical disinfectant (sodium hypochlorite) and improved LAD were tested on four-day-old (immature) biofilms. In stage 2, conventional LAD, improved LAD and chemomechanical disinfection (alone and in combination with improved LAD) were tested on four-week-old (mature) biofilms. RESULTS: Sodium hypochlorite and improved LAD showed the ability to significantly inactivate bacteria in four-day-old biofilms when compared to the control and LAD (p < 0.05). Inactivation of bacteria from deeper dentine was higher in improved LAD than sodium hypochlorite. In four-week-old biofilms, a combination of chemomechanical disinfection and improved LAD produced significant bacterial killing compared to either chemomechanical disinfection or improved LAD alone. CONCLUSIONS: This study highlighted the potential of improved LAD to kill bacteria within dentinal tubules. In combination with chemomechanical preparation, the improved LAD significantly inactivated four-week-old biofilm bacteria.
BACKGROUND: An improved light activated disinfection technique utilizing a specific photosensitizer formulation, liquid optical-conduit, oxygen-carrier and light energy of appropriate wavelength has been introduced recently. This study tested the efficacy of this improved light activated disinfection on ex vivo biofilms of Enterococcus faecalis at two different stages of maturation. METHODS: Eighty-five tooth sections were prepared and endodontic biofilm of E. faecalis were grown within the root canal. In stage 1, conventional light activated disinfection (LAD), chemical disinfectant (sodium hypochlorite) and improved LAD were tested on four-day-old (immature) biofilms. In stage 2, conventional LAD, improved LAD and chemomechanical disinfection (alone and in combination with improved LAD) were tested on four-week-old (mature) biofilms. RESULTS:Sodium hypochlorite and improved LAD showed the ability to significantly inactivate bacteria in four-day-old biofilms when compared to the control and LAD (p < 0.05). Inactivation of bacteria from deeper dentine was higher in improved LAD than sodium hypochlorite. In four-week-old biofilms, a combination of chemomechanical disinfection and improved LAD produced significant bacterial killing compared to either chemomechanical disinfection or improved LAD alone. CONCLUSIONS: This study highlighted the potential of improved LAD to kill bacteria within dentinal tubules. In combination with chemomechanical preparation, the improved LAD significantly inactivated four-week-old biofilm bacteria.
Authors: Anna Ossmann; Stefan Kranz; Guellmar Andre; Andrea Völpel; Volker Albrecht; Alfred Fahr; Bernd W Sigusch Journal: Clin Oral Investig Date: 2014-06-21 Impact factor: 3.573
Authors: Raymond Ng; Fiza Singh; Despina A Papamanou; Xiaoqing Song; Chitrang Patel; Colleen Holewa; Niraj Patel; Vanja Klepac-Ceraj; Carla R Fontana; Ralph Kent; Tom C Pagonis; Philip P Stashenko; Nikolaos S Soukos Journal: J Endod Date: 2011-02 Impact factor: 4.171
Authors: Lintian Yuan; Peijun Lyu; Ying-Ying Huang; Ning Du; Wei Qi; Michael R Hamblin; Yuguang Wang Journal: J Photochem Photobiol B Date: 2019-12-16 Impact factor: 6.252
Authors: Yan Xu; Mark J Young; Ricardo A Battaglino; Leslie R Morse; Carla Raquel Fontana; Tom C Pagonis; Ralph Kent; Nikolaos S Soukos Journal: J Endod Date: 2009-09-20 Impact factor: 4.171