Studies on isolated corneocytes.

Synopsis The function of the stratum corneum is dependent on the functions of its constituent parts. This paper focusses on corneocyte dimensions and the implications of change in dimension for function. A technique in which it is possible to obtain a value for corneocyte thickness is described. The changes in corneocyte dimensions with age and site and after topical applications are documented. Techniques which measure the rate of desquamation are also described. The rate of passive corneocyte loss has been estimated by counting the numbers of corneocytes shed into chambers fixed to the skin surface. In addition the corneocyte loss after a standardized rotational stimulus to the skin surface has been measured. Scanning and transmission electron microscope examination of corneocytes obtained by a scrub procedure is also described. A preliminary report on an immunological approach to the study of the biochemistry of corneocytes and stratum corneum is also included.