AIMS: Subclassification of rhabdomyosarcoma (RMS) has clinical relevance, as the two major subclasses embryonal (ERMS) and alveolar (ARMS) rhabdomyosarcoma differ greatly in terms of aggressiveness and prognosis. However, histological analysis is not always sufficient for an unequivocal subclassification of RMS. Furthermore, clinical presentation of ARMS has been reported to mimic other tumour types, specifically lymphoma. The aim was to determine the role of four biomarkers in the diagnosis of rhabdomyosarcoma. METHODS AND RESULTS: Recently, we identified four potential biomarkers to subclassify RMS with high sensitivity and specificity. These included epidermal growth factor receptor (EGFR) and fibrillin-2 as markers for ERMS, and AP2beta and P-cadherin as markers for translocation-positive ARMS. Here, we further validate the potential of these four markers in a second, independent patient cohort by immunohistochemistry on 80 sections of RMS biopsy specimens as well as a tissue microarray representing 18 different additional tumour types, including seven lymphomas. The combination of EGFR and fibrillin-2 was able to detect ERMS with a specificity of 76% and sensitivity of 90%. The combination of AP2beta and P-cadherin detected ARMS with a specificity of 97% and sensitivity of 90%, data very similar to our previous study. Furthermore, all lymphomas were clearly negative for AP2beta and P-cadherin. CONCLUSIONS: These four biomarkers are suitable for clinical implementation in the future diagnosis of RMS.
AIMS: Subclassification of rhabdomyosarcoma (RMS) has clinical relevance, as the two major subclasses embryonal (ERMS) and alveolar (ARMS) rhabdomyosarcoma differ greatly in terms of aggressiveness and prognosis. However, histological analysis is not always sufficient for an unequivocal subclassification of RMS. Furthermore, clinical presentation of ARMS has been reported to mimic other tumour types, specifically lymphoma. The aim was to determine the role of four biomarkers in the diagnosis of rhabdomyosarcoma. METHODS AND RESULTS: Recently, we identified four potential biomarkers to subclassify RMS with high sensitivity and specificity. These included epidermal growth factor receptor (EGFR) and fibrillin-2 as markers for ERMS, and AP2beta and P-cadherin as markers for translocation-positive ARMS. Here, we further validate the potential of these four markers in a second, independent patient cohort by immunohistochemistry on 80 sections of RMS biopsy specimens as well as a tissue microarray representing 18 different additional tumour types, including seven lymphomas. The combination of EGFR and fibrillin-2 was able to detect ERMS with a specificity of 76% and sensitivity of 90%. The combination of AP2beta and P-cadherin detected ARMS with a specificity of 97% and sensitivity of 90%, data very similar to our previous study. Furthermore, all lymphomas were clearly negative for AP2beta and P-cadherin. CONCLUSIONS: These four biomarkers are suitable for clinical implementation in the future diagnosis of RMS.
Authors: Erin R Rudzinski; James R Anderson; Elizabeth R Lyden; Julia A Bridge; Frederic G Barr; Julie M Gastier-Foster; Karen Bachmeyer; Stephen X Skapek; Douglas S Hawkins; Lisa A Teot; David M Parham Journal: Am J Surg Pathol Date: 2014-05 Impact factor: 6.394
Authors: Brian P Rubin; Koichi Nishijo; Hung-I Harry Chen; Xiaolan Yi; David P Schuetze; Ranadip Pal; Suresh I Prajapati; Jinu Abraham; Benjamin R Arenkiel; Qing-Rong Chen; Sean Davis; Amanda T McCleish; Mario R Capecchi; Joel E Michalek; Lee Ann Zarzabal; Javed Khan; Zhongxin Yu; David M Parham; Frederic G Barr; Paul S Meltzer; Yidong Chen; Charles Keller Journal: Cancer Cell Date: 2011-02-15 Impact factor: 31.743
Authors: Anand G Patel; Xiang Chen; Xin Huang; Michael R Clay; Natalia Komorova; Matthew J Krasin; Alberto Pappo; Heather Tillman; Brent A Orr; Justina McEvoy; Brittney Gordon; Kaley Blankenship; Colleen Reilly; Xin Zhou; Jackie L Norrie; Asa Karlstrom; Jiyang Yu; Dominik Wodarz; Elizabeth Stewart; Michael A Dyer Journal: Dev Cell Date: 2022-04-27 Impact factor: 13.417
Authors: Ken Kikuchi; Eri Taniguchi; Hung-I Harry Chen; Matthew N Svalina; Jinu Abraham; Elaine T Huang; Koichi Nishijo; Sean Davis; Christopher Louden; Lee Ann Zarzabal; Olivia Recht; Ayeza Bajwa; Noah Berlow; Mònica Suelves; Sherrie L Perkins; Paul S Meltzer; Atiya Mansoor; Joel E Michalek; Yidong Chen; Brian P Rubin; Charles Keller Journal: Skelet Muscle Date: 2013-11-25 Impact factor: 4.912