S Lakshmi1, P Rema, Thara Somanathan. 1. Division of Molecular Medicine, Regional Cancer Center, Thiruvananthapuram, India. rcc.lakshmi@gmail.com
Abstract
OBJECTIVE: To strengthen the role of high-risk human papillomaviruses (HPVs) in the development of cervical cancer, and the association between HPV and the cell cycle inhibitor gene p16ink4a in cervical carcinogenesis. METHODS: In this study, the association between p16ink4a and the presence of HPV DNA in cervical lesions was investigated in a total of 177 cervical biopsies classified as benign (n = 42), low-grade squamous intraepithelial lesions (LSIL; n = 34), high-grade squamous intraepithelial lesions (HSIL; n = 48) and invasive cancer (n = 53). p16ink4a expression was analyzed by immunohistochemistry while HPV DNA was localized by in situ hybridization. RESULTS: No significant association was found between p16ink4a expression and HPV presence in benign and LSIL samples. In HSIL and invasive cancer, a significant association was found between p16ink4a overexpression and the presence of HPV DNA. CONCLUSION: Immunohistochemical p16ink4a expression is associated with HPV infection in HSIL and cervical cancer, suggesting a role of p16 as a biomarker of HPV-associated cervical lesions. Copyright 2009 S. Karger AG, Basel.
OBJECTIVE: To strengthen the role of high-risk human papillomaviruses (HPVs) in the development of cervical cancer, and the association between HPV and the cell cycle inhibitor gene p16ink4a in cervical carcinogenesis. METHODS: In this study, the association between p16ink4a and the presence of HPV DNA in cervical lesions was investigated in a total of 177 cervical biopsies classified as benign (n = 42), low-grade squamous intraepithelial lesions (LSIL; n = 34), high-grade squamous intraepithelial lesions (HSIL; n = 48) and invasive cancer (n = 53). p16ink4a expression was analyzed by immunohistochemistry while HPV DNA was localized by in situ hybridization. RESULTS: No significant association was found between p16ink4a expression and HPV presence in benign and LSIL samples. In HSIL and invasive cancer, a significant association was found between p16ink4a overexpression and the presence of HPV DNA. CONCLUSION: Immunohistochemical p16ink4a expression is associated with HPV infection in HSIL and cervical cancer, suggesting a role of p16 as a biomarker of HPV-associated cervical lesions. Copyright 2009 S. Karger AG, Basel.
Authors: Anthony Mwololo; Joshua Nyagol; Emily Rogena; Willis Ochuk; Mary Kimani; Noel Onyango; Lorenzo Pacenti; Rosa Santopietro; Lorenzo Leoncini; Walter Mwanda Journal: Infect Agent Cancer Date: 2014-02-26 Impact factor: 2.965