| Literature DB >> 19467650 |
Hongtao Bi1, Xiuzhen Ni, Xiaoyu Liu, Jeff Iteku, Guihua Tai, Yifa Zhou, Jimin Zhao.
Abstract
A low molecular-weight polysaccharide named BIWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (M(w)/M(n)) was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear beta-(1-->6)-D-glucan without side chains. This is the first time to report a linear beta-(1-->6)-D-glucan with low molecular weight in non-lichenized ascomycete.Entities:
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Year: 2009 PMID： 19467650 DOI： 10.1016/j.carres.2009.04.009
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104