OBJECTIVE: Growth factors, including brain-derived neurotrophic factor (BDNF), are polypeptides that are involved in the maintenance, survival, and death of central and peripheral cells. Numerous growth factors have been identified in saliva and are thought to promote wound healing and maintenance of the oral epithelium. The aim of this study was to determine if BDNF is also found in human saliva. METHODS: Whole, unstimulated saliva samples (n = 30) were analyzed by SDS-PAGE and Western blot using an anti-human BDNF antibody. Proteolytic cleavage products were similarly assessed following the incubation of pooled saliva with N-glycanase F and plasmin. Subjects were also genotyped for the BDNF Val66Met single nucleotide polymorphism (SNP). RESULTS: These experiments revealed the presence of immunoreactive bands at 14, 32 and 34 kDa, corresponding to mature (mBDNF) and proBDNF, as well as a truncated pro-form at 24 kDa. Not every sample contained all forms of BDNF. Treatment with N-glycanase and plasmin reduced the size of the higher molecular weight bands, confirming the glycosylated pro-form of BDNF. mBDNF was detected significantly less often in subjects with the Val66Met SNP, compared to those without the polymorphism (chi(2) = 4.05; P < 0.05). CONCLUSIONS: While the function of salivary BDNF still requires elucidation, these findings suggest that it may be possible to use saliva in lieu of blood in future studies of BDNF and the Val66Met polymorphism.
OBJECTIVE: Growth factors, including brain-derived neurotrophic factor (BDNF), are polypeptides that are involved in the maintenance, survival, and death of central and peripheral cells. Numerous growth factors have been identified in saliva and are thought to promote wound healing and maintenance of the oral epithelium. The aim of this study was to determine if BDNF is also found in human saliva. METHODS: Whole, unstimulated saliva samples (n = 30) were analyzed by SDS-PAGE and Western blot using an anti-humanBDNF antibody. Proteolytic cleavage products were similarly assessed following the incubation of pooled saliva with N-glycanase F and plasmin. Subjects were also genotyped for the BDNFVal66Met single nucleotide polymorphism (SNP). RESULTS: These experiments revealed the presence of immunoreactive bands at 14, 32 and 34 kDa, corresponding to mature (mBDNF) and proBDNF, as well as a truncated pro-form at 24 kDa. Not every sample contained all forms of BDNF. Treatment with N-glycanase and plasmin reduced the size of the higher molecular weight bands, confirming the glycosylated pro-form of BDNF. mBDNF was detected significantly less often in subjects with the Val66Met SNP, compared to those without the polymorphism (chi(2) = 4.05; P < 0.05). CONCLUSIONS: While the function of salivary BDNF still requires elucidation, these findings suggest that it may be possible to use saliva in lieu of blood in future studies of BDNF and the Val66Met polymorphism.
Authors: Paul Denny; Fred K Hagen; Markus Hardt; Lujian Liao; Weihong Yan; Martha Arellanno; Sara Bassilian; Gurrinder S Bedi; Pinmannee Boontheung; Daniel Cociorva; Claire M Delahunty; Trish Denny; Jason Dunsmore; Kym F Faull; Joyce Gilligan; Mireya Gonzalez-Begne; Frédéric Halgand; Steven C Hall; Xuemei Han; Bradley Henson; Johannes Hewel; Shen Hu; Sherry Jeffrey; Jiang Jiang; Joseph A Loo; Rachel R Ogorzalek Loo; Daniel Malamud; James E Melvin; Olga Miroshnychenko; Mahvash Navazesh; Richard Niles; Sung Kyu Park; Akraporn Prakobphol; Prasanna Ramachandran; Megan Richert; Sarah Robinson; Melissa Sondej; Puneet Souda; Mark A Sullivan; Jona Takashima; Shawn Than; Jianghua Wang; Julian P Whitelegge; H Ewa Witkowska; Lawrence Wolinsky; Yongming Xie; Tao Xu; Weixia Yu; Jimmy Ytterberg; David T Wong; John R Yates; Susan J Fisher Journal: J Proteome Res Date: 2008-03-25 Impact factor: 4.466
Authors: Henry K Teng; Kenneth K Teng; Ramee Lee; Saundrene Wright; Seema Tevar; Ramiro D Almeida; Pouneh Kermani; Risa Torkin; Zhe-Yu Chen; Francis S Lee; Rosemary T Kraemer; Anders Nykjaer; Barbara L Hempstead Journal: J Neurosci Date: 2005-06-01 Impact factor: 6.167
Authors: Viktorija Trajkovska; Anders B Marcussen; Maj Vinberg; Per Hartvig; Susana Aznar; Gitte M Knudsen Journal: Brain Res Bull Date: 2007-04-09 Impact factor: 4.077