| Literature DB >> 19467445 |
Raymond Surzycki1, Katie Greenham, Kaoru Kitayama, Flora Dibal, Richard Wagner, Jean-David Rochaix, Tarek Ajam, Stefan Surzycki.
Abstract
PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.Entities:
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Year: 2009 PMID： 19467445 DOI： 10.1016/j.biologicals.2009.02.005
Source DB: PubMed Journal: Biologicals ISSN： 1045-1056 Impact factor: 1.856