Literature DB >> 19465523

Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria).

Qianqian Liu1, Bassem Allam, Jackie L Collier.   

Abstract

We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

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Year:  2009        PMID： 19465523      PMCID： PMC2708448          DOI： 10.1128/AEM.00246-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

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5.  Observations of a Protistan Disease Similar to QPX in Mercenaria mercenaria (Hard Clams) from the Coast of Massachusetts

Authors: 
Journal:  J Invertebr Pathol       Date:  1998-01       Impact factor: 2.841

6.  Structure of the large ribosomal subunit RNA of Phytophthora megasperma, and phylogeny of the oomycetes.

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Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

8.  Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria.

Authors:  N A Stokes; L M Ragone Calvo; K S Reece; E M Burreson
Journal:  Dis Aquat Organ       Date:  2002-12-10       Impact factor: 1.802

9.  Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate).

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Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

10.  Real-time PCR investigation of parasite ecology: in situ determination of oyster parasite Perkinsus marinus transmission dynamics in lower Chesapeake Bay.

Authors:  C Audemard; L M Ragone Calvo; K T Paynter; K S Reece; E M Burreson
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  2 in total

1.  Characterization of the transcriptome and temperature-induced differential gene expression in QPX, the thraustochytrid parasite of hard clams.

Authors:  Ewelina Rubin; Arnaud Tanguy; Mickael Perrigault; Emmanuelle Pales Espinosa; Bassem Allam
Journal:  BMC Genomics       Date:  2014-03-28       Impact factor: 3.969

2.  Clam focal and systemic immune responses to QPX infection revealed by RNA-seq technology.

Authors:  Kailai Wang; Carmelo del Castillo; Erwan Corre; Emmanuelle Pales Espinosa; Bassem Allam
Journal:  BMC Genomics       Date:  2016-02-27       Impact factor: 3.969
  2 in total

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