OBJECTIVES: To assess the performance of the Doumas bilirubin reference method. DESIGN AND METHODS: Ring trails using pooled patient specimens, a calibrator and human sera enriched with unconjugated bilirubin were analyzed in five laboratories using the Doumas bilirubin reference method. RESULTS: The coefficient of variation for the linear measurement range between laboratories ranged from 1-3%. CONCLUSIONS: The Doumas bilirubin reference method is robust and reproducible. Bilirubin results using this method may be used in the development of more accurate and reliable calibrators.
OBJECTIVES: To assess the performance of the Doumasbilirubin reference method. DESIGN AND METHODS: Ring trails using pooled patient specimens, a calibrator and human sera enriched with unconjugated bilirubin were analyzed in five laboratories using the Doumasbilirubin reference method. RESULTS: The coefficient of variation for the linear measurement range between laboratories ranged from 1-3%. CONCLUSIONS: The Doumasbilirubin reference method is robust and reproducible. Bilirubin results using this method may be used in the development of more accurate and reliable calibrators.