Literature DB >> 1946430

A regulated RNA binding protein also possesses aconitase activity.

S Kaptain1, W E Downey, C Tang, C Philpott, D Haile, D G Orloff, J B Harford, T A Rouault, R D Klausner.   

Abstract

A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.

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Year:  1991        PMID： 1946430      PMCID： PMC52877          DOI： 10.1073/pnas.88.22.10109

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Structural relationship between an iron-regulated RNA-binding protein (IRE-BP) and aconitase: functional implications.

Authors:  T A Rouault; C D Stout; S Kaptain; J B Harford; R D Klausner
Journal:  Cell       Date:  1991-03-08       Impact factor: 41.582

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4.  Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein.

Authors:  T A Rouault; C K Tang; S Kaptain; W H Burgess; D J Haile; F Samaniego; O W McBride; J B Harford; R D Klausner
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

5.  19th Sir Hans Krebs lecture. Engineering of protein bound iron-sulfur clusters. A tool for the study of protein and cluster chemistry and mechanism of iron-sulfur enzymes.

Authors:  H Beinert; M C Kennedy
Journal:  Eur J Biochem       Date:  1989-12-08

6.  Regulation of interaction of the iron-responsive element binding protein with iron-responsive RNA elements.

Authors:  D J Haile; M W Hentze; T A Rouault; J B Harford; R D Klausner
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

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Authors:  T B Shows; J A Brown
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Authors:  J Rogers; H Munro
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

9.  Cloning and structural characterization of porcine heart aconitase.

Authors:  L Zheng; P C Andrews; M A Hermodson; J E Dixon; H Zalkin
Journal:  J Biol Chem       Date:  1990-02-15       Impact factor: 5.157

10.  Hemin, chelatable iron, and the regulation of transferrin receptor biosynthesis.

Authors:  T Rouault; K Rao; J Harford; E Mattia; R D Klausner
Journal:  J Biol Chem       Date:  1985-11-25       Impact factor: 5.157
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  51 in total

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8.  The iron-responsive element-binding protein: localization of the RNA-binding site to the aconitase active-site cleft.

Authors:  J P Basilion; T A Rouault; C M Massinople; R D Klausner; W H Burgess
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

9.  A novel method to identify nucleic acid binding sites in proteins by scanning mutagenesis: application to iron regulatory protein.

Authors:  B Neupert; E Menotti; L C Kühn
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10.  A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.

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