OBJECTIVE: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation. DESIGN: Prospective study. SETTING: Research institute. PATIENTS: Women undergoing laparoscopic sterilization. INTERVENTION(S): Small antral follicle cumulus-oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E(2)), serum replacement (SR), BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated. MAIN OUTCOME MEASURE(S): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAlP6, PTGS2, GREM1) by reverse transcription-polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development. RESULT(S): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68%; TrkB/Fc 57%). Failure to cleave was highest in H + BDNF + SR (92%). Only H + SR yielded morulae/blastocysts (6%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation. CONCLUSION(S): The BDNF signaling within COCs influences oocyte maturation and early embryogenesis. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation. DESIGN: Prospective study. SETTING: Research institute. PATIENTS: Women undergoing laparoscopic sterilization. INTERVENTION(S): Small antral follicle cumulus-oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E(2)), serum replacement (SR), BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated. MAIN OUTCOME MEASURE(S): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAlP6, PTGS2, GREM1) by reverse transcription-polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development. RESULT(S): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68%; TrkB/Fc 57%). Failure to cleave was highest in H + BDNF + SR (92%). Only H + SR yielded morulae/blastocysts (6%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation. CONCLUSION(S): The BDNF signaling within COCs influences oocyte maturation and early embryogenesis. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Sohyun L McElroy; James A Byrne; Shawn L Chavez; Barry Behr; Aaron J Hsueh; Lynn M Westphal; Renee A Reijo Pera Journal: PLoS One Date: 2010-06-07 Impact factor: 3.240
Authors: Shawn L Chavez; Sohyun L McElroy; Nancy L Bossert; Christopher J De Jonge; Maria Vera Rodriguez; Denise E Leong; Barry Behr; Lynn M Westphal; Renee A Reijo Pera Journal: Hum Mol Genet Date: 2014-05-12 Impact factor: 6.150