OBJECTIVES: The aim of the study was to determine the modifications of the mutational archive in proviral HIV-1 DNA occurring during 24 months of intermittent or continuous highly active antiretroviral therapy (HAART). METHODS: The study population included subjects enrolled in the Istituto Superiore di Sanità Pulsed Antiretroviral Therapy (ISS PART) clinical trial. All of these patients were on first-line HAART and had plasma HIV-1 RNA below 50 HIV-1 RNA copies/mL. A genotypic resistance test was performed on HIV-1 DNA extracted from peripheral blood mononuclear cells (PBMC) at baseline and after 24 months of follow-up. Resistance-associated mutations (RAMs) were defined according to the International AIDS Society (IAS) USA classification. RESULTS:Sixty-nine subjects were included in the study [36 enrolled in arm A of the ISS PART (continuous HAART) and 33 enrolled in arm B (intermittent HAART)]. No major modifications of the mutational archive were found in either group after 24 months of follow-up, in terms of both the proportion of subjects with mutations and the total number of mutations. CONCLUSIONS: In this patient population, the mutational archive in HIV-1 DNA extracted from PBMC was stable for 24 months, irrespective of HAART modality, whether continuous or intermittent.
RCT Entities:
OBJECTIVES: The aim of the study was to determine the modifications of the mutational archive in proviral HIV-1 DNA occurring during 24 months of intermittent or continuous highly active antiretroviral therapy (HAART). METHODS: The study population included subjects enrolled in the Istituto Superiore di Sanità Pulsed Antiretroviral Therapy (ISS PART) clinical trial. All of these patients were on first-line HAART and had plasma HIV-1 RNA below 50 HIV-1 RNA copies/mL. A genotypic resistance test was performed on HIV-1 DNA extracted from peripheral blood mononuclear cells (PBMC) at baseline and after 24 months of follow-up. Resistance-associated mutations (RAMs) were defined according to the International AIDS Society (IAS) USA classification. RESULTS: Sixty-nine subjects were included in the study [36 enrolled in arm A of the ISS PART (continuous HAART) and 33 enrolled in arm B (intermittent HAART)]. No major modifications of the mutational archive were found in either group after 24 months of follow-up, in terms of both the proportion of subjects with mutations and the total number of mutations. CONCLUSIONS: In this patient population, the mutational archive in HIV-1 DNA extracted from PBMC was stable for 24 months, irrespective of HAART modality, whether continuous or intermittent.
Authors: Anne Derache; Hyoung-Shik Shin; Maya Balamane; Elizabeth White; Dennis Israelski; Jeffrey D Klausner; Alexandra H Freeman; David Katzenstein Journal: PLoS One Date: 2015-01-30 Impact factor: 3.240
Authors: Vijay Bansode; Grace P McCormack; Amelia C Crampin; Bagrey Ngwira; Ram K Shrestha; Neil French; Judith R Glynn; Simon A Travers Journal: BMC Infect Dis Date: 2013-01-30 Impact factor: 3.090
Authors: A T Makadzange; M Higgins-Biddle; B Chimukangara; R Birri; M Gordon; T Mahlanza; G McHugh; J H van Dijk; M Bwakura-Dangarembizi; T Ndung'u; C Masimirembwa; B Phelps; A Amzel; B O Ojikutu; B D Walker; C E Ndhlovu Journal: PLoS One Date: 2015-12-14 Impact factor: 3.240