AIM: Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation. MATERIALS AND METHODS: Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used. RESULTS: In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2--in 46.03%, CMV--in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women. CONCLUSION: Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.
AIM: Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation. MATERIALS AND METHODS: Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used. RESULTS: In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2--in 46.03%, CMV--in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women. CONCLUSION: Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.
Authors: S A Batwa; A M Ashshi; F F Kamfar; J Ahmad; S Idris; A Khojah; N M Al-Qadi; B Refaat Journal: Eur J Clin Microbiol Infect Dis Date: 2015-11-12 Impact factor: 3.267