Literature DB >> 19459071

Treatment of injured neurons with bone marrow stem cells cotransfected by hTERT and Ad-BDNF in vitro.

Zhitao Wang1, Quanjun Deng, Xiaohui Zhang, Jianning Zhang.   

Abstract

The purpose of this paper is to study the anti-injury effect of bone marrow stromal cells (BMSCs) transfected by human telomerase reverse transcriptase (hTERT) and adenovirous brain-derived neurotrophic factor (Ad-BDNF) in injured neurons. Rat injured neurons were induced in vitro and then cocultured with rBMSCs transfected by hTERT and Ad-BDNF. The anti-injury effect of rBMSCs transfected by hTERT and Ad-BDNF was detected by Western blot for neural apoptosis gene MAP2 and NF-kappaB and lactic dehydrogenase released by injured neurons. Our data demonstrated that graft rBMSCs transfected by hTERT and Ad-BDNF could increase the expression of BDNF in injured neurons and alleviate apoptosis of the injured neurons. According to these data, BDNF-BMSCs demonstrate a tendency to be protection against neuronal death caused by apoptosis which would last a long time after neural injury. The data also show the potential of BDNF-BMSCs as a promising therapeutic strategy that warrants further investigation in patients with traumatic brain injury.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19459071     DOI： 10.1007/s12031-009-9208-5

Source DB:  PubMed          Journal:  J Mol Neurosci        ISSN： 0895-8696            Impact factor:   3.444


  42 in total

1.  Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms.

Authors:  T Kinnaird; E Stabile; M S Burnett; C W Lee; S Barr; S Fuchs; S E Epstein
Journal:  Circ Res       Date:  2004-01-22       Impact factor: 17.367

2.  Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal cord.

Authors:  Sufan Wu; Yoshihisa Suzuki; Yoko Ejiri; Toru Noda; Hongliang Bai; Masaaki Kitada; Kazuya Kataoka; Masayoshi Ohta; Hirotomi Chou; Chizuka Ide
Journal:  J Neurosci Res       Date:  2003-05-01       Impact factor: 4.164

3.  Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.

Authors:  Victor Vacanti; Elton Kong; Gen Suzuki; Kazuki Sato; John M Canty; Techung Lee
Journal:  J Cell Physiol       Date:  2005-11       Impact factor: 6.384

4.  Intraarterial administration of marrow stromal cells in a rat model of traumatic brain injury.

Authors:  D Lu; Y Li; L Wang; J Chen; A Mahmood; M Chopp
Journal:  J Neurotrauma       Date:  2001-08       Impact factor: 5.269

5.  High tolerance and delayed elastic response of cultured axons to dynamic stretch injury.

Authors:  D H Smith; J A Wolf; T A Lusardi; V M Lee; D F Meaney
Journal:  J Neurosci       Date:  1999-06-01       Impact factor: 6.167

6.  Adult bone marrow stromal cells differentiate into neural cells in vitro.

Authors:  J Sanchez-Ramos; S Song; F Cardozo-Pelaez; C Hazzi; T Stedeford; A Willing; T B Freeman; S Saporta; W Janssen; N Patel; D R Cooper; P R Sanberg
Journal:  Exp Neurol       Date:  2000-08       Impact factor: 5.330

7.  Effects of telomerase and telomere length on epidermal stem cell behavior.

Authors:  Ignacio Flores; María L Cayuela; María A Blasco
Journal:  Science       Date:  2005-07-21       Impact factor: 47.728

8.  A novel role for serum response factor in neuronal survival.

Authors:  Sandra H Chang; Steve Poser; Zhengui Xia
Journal:  J Neurosci       Date:  2004-03-03       Impact factor: 6.167

9.  Ischemic rat brain extracts induce human marrow stromal cell growth factor production.

Authors:  Xiaoguang Chen; Yi Li; Lei Wang; Mark Katakowski; Lijie Zhang; Jieli Chen; Yongxian Xu; Subhash C Gautam; Michael Chopp
Journal:  Neuropathology       Date:  2002-12       Impact factor: 1.906

10.  Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer.

Authors:  Wolfgang Böcker; Zhanhai Yin; Inga Drosse; Florian Haasters; Oliver Rossmann; Matthias Wierer; Cvetan Popov; Melanie Locher; Wolf Mutschler; Denitsa Docheva; Matthias Schieker
Journal:  J Cell Mol Med       Date:  2008-03-04       Impact factor: 5.310
View more
  4 in total

1.  Protective effects of BDNF overexpression bone marrow stromal cell transplantation in rat models of traumatic brain injury.

Authors:  Zhitao Wang; Weifeng Yao; Quanjun Deng; Xiaohui Zhang; Jianning Zhang
Journal:  J Mol Neurosci       Date:  2012-11-11       Impact factor: 3.444

2.  Overexpression of β-NGF promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathway.

Authors:  Jun Yuan; Guorong Huang; Zhe Xiao; Lvbiao Lin; Tianwang Han
Journal:  Mol Cell Biochem       Date:  2013-08-10       Impact factor: 3.396

3.  Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Authors:  Yang Hu; Yan Zhang; Kang Tian; Chong Xun; Shouyu Wang; Decheng Lv
Journal:  Mol Med Rep       Date:  2015-11-11       Impact factor: 2.952

4.  NGF and TERT co-transfected BMSCs improve the restoration of cognitive impairment in vascular dementia rats.

Authors:  Fei Wang; Guangming Chang; Xin Geng
Journal:  PLoS One       Date:  2014-06-02       Impact factor: 3.240
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.