| Literature DB >> 19458047 |
Federico Gonzalez1, Montserrat Barragan Monasterio, Gustavo Tiscornia, Nuria Montserrat Pulido, Rita Vassena, Laura Batlle Morera, Ignasi Rodriguez Piza, Juan Carlos Izpisua Belmonte.
Abstract
Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.Entities:
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Year: 2009 PMID： 19458047 PMCID： PMC2690033 DOI： 10.1073/pnas.0901471106
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205