| Literature DB >> 19457428 |
Yun-Gon Kim1, Hee-Jin Jeong, Kyoung-Soon Jang, Yung-Hun Yang, Yong-Sang Song, Junho Chung, Byung-Gee Kim.
Abstract
We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.Entities:
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Year: 2009 PMID: 19457428 DOI: 10.1016/j.ab.2009.05.015
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365