The pathology of necrotic enteritis of chickens following infusion of broth cultures of Clostridium perfringens into the duodenum.

Necrotic enteritis was consistently reproduced when enough active broth culture of Clostridium perfringens type A was infused intraduodenally. Typical lesions of necrotic enteritis were seen as early as 5 hr after infusion was begun. The histologic lesions observed at 1 hr were characterized by edema in the lamina propria and desquamation of epithelial cells. Large numbers of clostridia were seen among these sloughed cells. Coagulation necrosis of the tips of villi became evident at 3 hr and was marked at 5 hr. Many clumps of clostridia were obvious among the necrotic tissue. At 8 and 12 hr the necrotic lesions extended to involve most of the villus structures. Morphologically abnormal erythrocytes were evident in the visceral organs at 12 hr.