BACKGROUND: There is a growing body of evidence indicating that epigenetic influences originating from stromal cells in the immediate microenvironment may play a role in carcinogenesis. Determining the molecular mechanisms involved in stromal-stem cell interaction could provide critical insight into prostate development and disease progression, particularly with regard to their relationship to and influence on the putative cancer stem cell. METHODS: Prostate and bladder stromal cells prepared from tissue specimens were co-cultured with the pluripotent embryonal carcinoma cell line NCCIT. Transcriptome analysis was used to characterize NCCIT cell response to prostate or bladder signaling. RESULTS: A systems approach demonstrated that prostate stromal cells were capable of inducing gene expression changes in NCCIT through secreted factors. Induction led to a loss of embryonic stem cell markers, with concurrent up-regulation of many genes characteristic of stromal mesenchyme cells as well as some of epithelial and cancer stem cells. Bladder stromal signaling produced gene expression changes different from those of prostate signaling. CONCLUSIONS: This study indicates that paracrine stromal cell signaling can affect cancer stem cell response in an organ-specific manner and may provide insight for future development of treatment strategies such as differentiation therapy.
BACKGROUND: There is a growing body of evidence indicating that epigenetic influences originating from stromal cells in the immediate microenvironment may play a role in carcinogenesis. Determining the molecular mechanisms involved in stromal-stem cell interaction could provide critical insight into prostate development and disease progression, particularly with regard to their relationship to and influence on the putative cancer stem cell. METHODS: Prostate and bladder stromal cells prepared from tissue specimens were co-cultured with the pluripotent embryonal carcinoma cell line NCCIT. Transcriptome analysis was used to characterize NCCIT cell response to prostate or bladder signaling. RESULTS: A systems approach demonstrated that prostate stromal cells were capable of inducing gene expression changes in NCCIT through secreted factors. Induction led to a loss of embryonic stem cell markers, with concurrent up-regulation of many genes characteristic of stromal mesenchyme cells as well as some of epithelial and cancer stem cells. Bladder stromal signaling produced gene expression changes different from those of prostate signaling. CONCLUSIONS: This study indicates that paracrine stromal cell signaling can affect cancer stem cell response in an organ-specific manner and may provide insight for future development of treatment strategies such as differentiation therapy.
Authors: Alvin Y Liu; Ricardo Z N Vêncio; Laura S Page; Melissa E Ho; Michelle A Loprieno; Lawrence D True Journal: Cell Tissue Res Date: 2012-03-20 Impact factor: 5.249
Authors: Gisely T Borges; Eneida F Vêncio; Ricardo Z N Vêncio; Robert L Vessella; Carol B Ware; Alvin Y Liu Journal: Curr Urol Rep Date: 2015-01 Impact factor: 3.092
Authors: Laura E Pascal; Junkui Ai; Ricardo Z N Vêncio; Eneida F Vêncio; Yong Zhou; Laura S Page; Lawrence D True; Zhou Wang; Alvin Y Liu Journal: Cancer Microenviron Date: 2011-01-07
Authors: Eneida F Vêncio; Laura E Pascal; Laura S Page; Gareth Denyer; Amy J Wang; Hannele Ruohola-Baker; Shile Zhang; Kai Wang; David J Galas; Alvin Y Liu Journal: J Cell Physiol Date: 2011-06 Impact factor: 6.384
Authors: Laura E Pascal; Young Ah Goo; Ricardo Zn Vêncio; Laura S Page; Amber A Chambers; Emily S Liebeskind; Thomas K Takayama; Lawrence D True; Alvin Y Liu Journal: BMC Cancer Date: 2009-09-08 Impact factor: 4.430
Authors: Laura E Pascal; Ricardo Zn Vêncio; Robert L Vessella; Carol B Ware; Eneida F Vêncio; Gareth Denyer; Alvin Y Liu Journal: BMC Med Genomics Date: 2011-05-23 Impact factor: 3.063