BACKGROUND: Intoxications caused by amanitin-containing mushrooms represent an unresolved problem in clinical toxicology. The objective of this study was a comparative evaluation of benzylpenicillin (Bp), acetylcysteine (ACC) and silibinin (Sil) efficacy as antidotes in hepatocytes intoxicated with alpha-amanitin (alpha-AMA). MATERIALS AND METHODS: All experiments were performed on cultured canine hepatocytes. Cytotoxicity evaluation of cultured cells (MTT assay, extracellular lactate dehydrogenase activity) was performed at 12, 24 and 48 h of exposure to alpha-AMA and/or antidotes. RESULTS: Following 24 and 48 h exposure there was a significant decline of hepatocyte viability and an increase of lactate dehydrogenase activity in groups exposed to alpha-AMA and in groups exposed simultaneously to alpha-AMA and antidotes. Moreover, hepatocyte viability and lactate dehydrogenase activity in all these groups were similar. Administration of studied antidotes without alpha-AMA, was not associated with any adverse effects in hepatocytes. CONCLUSION: All antidotes tested in this study against alpha-AMA were not effective in canine hepatocyte cultures.
BACKGROUND: Intoxications caused by amanitin-containing mushrooms represent an unresolved problem in clinical toxicology. The objective of this study was a comparative evaluation of benzylpenicillin (Bp), acetylcysteine (ACC) and silibinin (Sil) efficacy as antidotes in hepatocytes intoxicated with alpha-amanitin (alpha-AMA). MATERIALS AND METHODS: All experiments were performed on cultured canine hepatocytes. Cytotoxicity evaluation of cultured cells (MTT assay, extracellular lactate dehydrogenase activity) was performed at 12, 24 and 48 h of exposure to alpha-AMA and/or antidotes. RESULTS: Following 24 and 48 h exposure there was a significant decline of hepatocyte viability and an increase of lactate dehydrogenase activity in groups exposed to alpha-AMA and in groups exposed simultaneously to alpha-AMA and antidotes. Moreover, hepatocyte viability and lactate dehydrogenase activity in all these groups were similar. Administration of studied antidotes without alpha-AMA, was not associated with any adverse effects in hepatocytes. CONCLUSION: All antidotes tested in this study against alpha-AMA were not effective in canine hepatocyte cultures.
Authors: Andrea Marchegiani; Alessandro Fruganti; Alessandra Gavazza; Sara Mangiaterra; Alessia Candellone; Eleonora Fusi; Giacomo Rossi; Matteo Cerquetella Journal: Vet Med Int Date: 2020-05-09
Authors: Abdulsamet Erden; Kübra Esmeray; Hatice Karagöz; Samet Karahan; Hasan Hüseyin Gümüşçü; Mustafa Başak; Ali Cetinkaya; Deniz Avcı; Orhan Kürşat Poyrazoğlu Journal: Int Med Case Rep J Date: 2013-11-22