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Literature DB >> 19454252

Recombination between linear double-stranded DNA substrates in vivo.

Kumaran Narayanan¹, Edmund Ui-Hang Sim, Nikolai V Ravin, Choon-Weng Lee.

Abstract

Recombineering technology in Escherichia coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains intact without undergoing rearrangements after recombination. This system will be valuable for direct in vivo manipulation of large linear DNA including the N15 and PY54 prophages and linear animal viruses, and for assembly of linear constructs as artificial chromosome vectors.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
DNA, Bacterial
DNA

Year: 2009 PMID： 19454252 PMCID： PMC2784165 DOI： 10.1016/j.ab.2009.01.015

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

16 in total

1. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

Authors: E C Lee; D Yu; J Martinez de Velasco; L Tessarollo; D A Swing; D L Court; N A Jenkins; N G Copeland
Journal: Genomics Date: 2001-04-01 Impact factor: 5.736

2. Intact recombineering of highly repetitive DNA requires reduced induction of recombination enzymes and improved host viability.

Authors: Kumaran Narayanan
Journal: Anal Biochem Date: 2008-01-24 Impact factor: 3.365

3. DNA cloning by homologous recombination in Escherichia coli.

Authors: Y Zhang; J P Muyrers; G Testa; A F Stewart
Journal: Nat Biotechnol Date: 2000-12 Impact factor: 54.908

4. Transgenic analysis of a 100-kb human beta-globin cluster-containing DNA fragment propagated as a bacterial artificial chromosome.

Authors: R M Kaufman; C T Pham; T J Ley
Journal: Blood Date: 1999-11-01 Impact factor: 22.113

Recombination between linear double-stranded DNA substrates in vivo.

1. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

2. Intact recombineering of highly repetitive DNA requires reduced induction of recombination enzymes and improved host viability.

3. DNA cloning by homologous recombination in Escherichia coli.

4. Transgenic analysis of a 100-kb human beta-globin cluster-containing DNA fragment propagated as a bacterial artificial chromosome.

5. P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster.

6. DNA modification and functional delivery into human cells using Escherichia coli DH10B.

7. Recombineering linear DNA that replicate stably in E. coli.

8. Interplay between the temperate phages PY54 and N15, linear plasmid prophages with covalently closed ends.

9. A new logic for DNA engineering using recombination in Escherichia coli.

Review 10. Mining and engineering natural-product biosynthetic pathways.

Review 1. Back to BAC: the use of infectious clone technologies for viral mutagenesis.

2. Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis.