Literature DB >> 19454240

Automated soft agar assay for the high-throughput screening of anticancer compounds.

René Thierbach1, Pablo Steinberg.   

Abstract

We have established an automated soft agar colony formation assay that can be used as a potent tool in experimental tumor therapy studies as well as anticancer compound screening. It allows the direct and simultaneous comparison of the effects of a high number of anticancer compounds on the anchorage-independent growth of a variety of tumor cell lines. By making use of a commercially available automated pipetting system, the user gets results of excellent quality within 1 week and does not need special cell culture practice.

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Year:  2009        PMID： 19454240     DOI： 10.1016/j.ab.2009.01.029

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

Review 1.  The production of 3D tumor spheroids for cancer drug discovery.

Authors:  Shilpa Sant; Paul A Johnston
Journal:  Drug Discov Today Technol       Date:  2017-04-14

2.  Inhibition of alanine aminotransferase in silico and in vivo promotes mitochondrial metabolism to impair malignant growth.

Authors:  Gregor Beuster; Kim Zarse; Christoph Kaleta; René Thierbach; Michael Kiehntopf; Pablo Steinberg; Stefan Schuster; Michael Ristow
Journal:  J Biol Chem       Date:  2011-05-03       Impact factor: 5.157

3.  Optimization of Invasion-Specific Effects of Betulin Derivatives on Prostate Cancer Cells through Lead Development.

Authors:  Ville Härmä; Raisa Haavikko; Johannes Virtanen; Ilmari Ahonen; Hannu-Pekka Schukov; Sami Alakurtti; Enkhee Purev; Heiko Rischer; Jari Yli-Kauhaluoma; Vânia M Moreira; Matthias Nees; Kirsi-Marja Oksman-Caldentey
Journal:  PLoS One       Date:  2015-05-12       Impact factor: 3.240

4.  Repair of Torn Avascular Meniscal Cartilage Using Undifferentiated Autologous Mesenchymal Stem Cells: From In Vitro Optimization to a First-in-Human Study.

Authors:  Michael R Whitehouse; Nicholas R Howells; Michael C Parry; Eric Austin; Wael Kafienah; Kyla Brady; Allen E Goodship; Jonathan D Eldridge; Ashley W Blom; Anthony P Hollander
Journal:  Stem Cells Transl Med       Date:  2016-12-15       Impact factor: 6.940

5.  Identifying chemopreventive agents for obesity-associated cancers using an efficient, 3D high-throughput transformation assay.

Authors:  Vanessa Benham; Blair Bullard; Thomas S Dexheimer; Matthew P Bernard; Richard R Neubig; Karen T Liby; Jamie J Bernard
Journal:  Sci Rep       Date:  2019-07-16       Impact factor: 4.379

6.  An inducible CRISPR/Cas9 screen identifies DTX2 as a transcriptional regulator of human telomerase.

Authors:  Zhifen Zhou; Yujing Li; Huimin Xu; Xiaowei Xie; Zibin He; Song Lin; Ruofei Li; Shouheng Jin; Jun Cui; Hai Hu; Feng Liu; Su Wu; Wenbin Ma; Zhou Songyang
Journal:  iScience       Date:  2022-01-25

7.  Advanced cell culture techniques for cancer drug discovery.

Authors:  Carrie J Lovitt; Todd B Shelper; Vicky M Avery
Journal:  Biology (Basel)       Date:  2014-05-30
  7 in total

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