| Literature DB >> 19454221 |
Tokuji Tsuchiya1, Nanako Kameya, Ikuo Nakamura.
Abstract
Polymerase chain reaction (PCR)-based genome walking techniques are commonly used to clone unknown genomic regions flanking known sequences. However, these methods are typically problematic when applied to highly complex DNA templates isolated from plants with large genomes. Here we describe a reliable and efficient genome walking method that is particularly effective for plants with large genomes. Our ligation-mediated PCR method, Straight Walk, has improved sensitivity and specificity due to optimization of sequences of adaptors and adaptor primers. Successful genome walking in lily, which has one of the largest genomes in plants, indicates that Straight Walk is applicable for most plant species.Mesh：
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Year: 2009 PMID： 19454221 DOI： 10.1016/j.ab.2009.02.002
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365