Anna Granath1, Rolf Uddman, Lars-Olaf Cardell. 1. Division of ENT Diseases, Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden. anna.granath@karolinska.se
Abstract
CONCLUSION: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). OBJECTIVES: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. SUBJECTS AND METHODS: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. RESULTS: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4.
CONCLUSION:Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). OBJECTIVES: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. SUBJECTS AND METHODS: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. RESULTS: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4.
Authors: Michael T Cheeseman; Hayley E Tyrer; Debbie Williams; Tertius A Hough; Paras Pathak; Maria R Romero; Helen Hilton; Sulzhan Bali; Andrew Parker; Lucie Vizor; Tom Purnell; Kate Vowell; Sara Wells; Mahmood F Bhutta; Paul K Potter; Steve D M Brown Journal: PLoS Genet Date: 2011-10-20 Impact factor: 5.917