A rabbit model to study biochemical damage to the lens after vitrectomy: effects of N-acetylcysteine.

The purpose of the present study was to determine whether the biochemical effects of vitrectomy can be studied in rabbits and to assess the possible protective effects of N-acetylcysteine on the lens following vitrectomy. Twenty-four New Zealand rabbits (2.3-2.4 kg) were divided into three groups of eight each. Left eyes underwent vitrectomy surgery. Unoperated right eyes served as controls. Equal numbers of treated eyes were not injected, injected with 20 mM N-acetylcysteine, or 100 mM N-acetylcysteine immediately after vitrectomy. Lens transparency was monitored by slit-lamp biomicroscopy pre- and post-vitrectomy. A series of biochemical measurements were performed on lenses five months after vitrectomy. No significant differences in lens transparency or structure were observed in the three groups of lenses. However, vitrectomy was associated with significantly decreased activity of Na(+)-K(+)-ATPase and catalase. Compared with the group not treated with N-acetylcysteine, catalase activity was increased significantly in the group treated with 20 mM N-acetylcysteine. The level of glutathione and the activities of Na(+)-K(+)-ATPase and glutathione reductase were also higher in the two N-acetylcysteine-treated groups than in the untreated group, although these differences did not reach statistical significance. For all measured parameters, the effect of 20 mM N-acetylcysteine appeared to be better than 100 mM N-acetylcysteine, although these differences were not statistically significant. From these results, we gather that vitrectomy is associated with long-term decreases in enzyme activity in the lens. Injection of N-acetylcysteine into the vitreous cavity protects against some of these changes. Antioxidants like N-acetylcysteine may slow or prevent post-vitrectomy cataracts.