Cheng Wang1, Zi-Li You, Ding-Ding Zhang. 1. Key Laboratory for Neuroinformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Abstract
OBJECTIVE: To investigate the expression of amyloid beta precursor-like protein 1(APLP1) gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats. METHODS: Epileptic rats were developed by LiC1 (3 mmol/kg, i.p.) approximately 20 h prior to pilocarpine (30 mg/kg, i.p.) administration. The 3' end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR. RESULTS: The 3'end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation of APLP1 mRNA expression at 6 h after epilepsy initiation (P< 0.05), and at 30 h, this down-regulation became more dramatic (P< 0.01), which lasted till day 15 (P< 0.01). CONCLUSION: The 3' end of APLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.
OBJECTIVE: To investigate the expression of amyloid beta precursor-like protein 1(APLP1) gene on the transcription level in hippocampus of pilocarpine-induced epilepticrats. METHODS:Epilepticrats were developed by LiC1 (3 mmol/kg, i.p.) approximately 20 h prior to pilocarpine (30 mg/kg, i.p.) administration. The 3' end partial sequence of ratAPLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epilepticrats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR. RESULTS: The 3'end partial sequence of ratAPLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation of APLP1 mRNA expression at 6 h after epilepsy initiation (P< 0.05), and at 30 h, this down-regulation became more dramatic (P< 0.01), which lasted till day 15 (P< 0.01). CONCLUSION: The 3' end of APLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epilepticrats.
Authors: Asla Pitkänen; Irina Kharatishvili; Heli Karhunen; Katarzyna Lukasiuk; Riikka Immonen; Jaak Nairismägi; Olli Gröhn; Jari Nissinen Journal: Epilepsia Date: 2007 Impact factor: 5.864
Authors: Paul Preece; David J Virley; Moheb Costandi; Robert Coombes; Stephen J Moss; Anne W Mudge; Elena Jazin; Nigel J Cairns Journal: Brain Res Mol Brain Res Date: 2004-03-17