ETHNOPHARMACOLOGICAL RELEVANCE: A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the "Qi" and nourish the "Blood". However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied. AIMS: This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis. MATERIALS AND METHODS: A myelosuppression mouse model was treated with DBT (10mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7, 14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3, and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e. RESULTS: Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells. CONCLUSION: DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes; (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes.
ETHNOPHARMACOLOGICAL RELEVANCE: A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the "Qi" and nourish the "Blood". However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied. AIMS: This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis. MATERIALS AND METHODS: A myelosuppressionmouse model was treated with DBT (10mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7, 14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3, and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e. RESULTS: Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells. CONCLUSION:DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes; (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes.
Authors: Jie Yu Ye; Godfrey Chi Fung Chan; Liang Qiao; Qizhou Lian; Fan Yi Meng; Xue Qun Luo; Levon M Khachigian; Ming Ma; Ruixia Deng; Jian Liang Chen; Beng H Chong; Mo Yang Journal: Haematologica Date: 2010-06-18 Impact factor: 9.941
Authors: Chang Liu; Jianqin Li; Fan Yi Meng; Simon X Liang; Ruixia Deng; Chi Kong Li; N H Pong; Ching Po Lau; Sau Wan Cheng; Jie Yu Ye; Jian L Chen; S T Yang; Haixia Yan; Shilin Chen; Beng H Chong; Mo Yang Journal: BMC Complement Altern Med Date: 2010-12-21 Impact factor: 3.659
Authors: Ji-Yan Su; Qing-Feng Xie; Wei-Jin Liu; Ping Lai; Dan-Dan Liu; Li-Hai Tang; Tina T X Dong; Zi-Ren Su; Karl W K Tsim; Xiao-Ping Lai; Kun-Yin Li Journal: Evid Based Complement Alternat Med Date: 2013-12-16 Impact factor: 2.629
Authors: You Yeon Choi; Mi Hye Kim; Jongki Hong; Kyuseok Kim; Woong Mo Yang Journal: Evid Based Complement Alternat Med Date: 2016-03-08 Impact factor: 2.629