| Literature DB >> 19443030 |
Miwako Narita1, Norihiro Watanabe, Akie Yamahira, Shigeo Hashimoto, Nozomi Tochiki, Anri Saitoh, Masami Kaji, Takeshi Nakamura, Tatsuo Furukawa, Ken Toba, Ichiro Fuse, Yoshifusa Aizawa, Masuhiro Takahashi.
Abstract
We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL.Entities:
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Year: 2009 PMID： 19443030 DOI： 10.1016/j.leukres.2009.03.047
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156