Philippe Taupin1. 1. Dublin City University, School of Biotechnology, Glasnevin, Dublin 9, Ireland. philippe.taupin@dcu.ie
Abstract
BACKGROUND: The application is in the field of embryonic stem cells (ESCs) and the cryopreservation of neuronal cells in culture. OBJECTIVE: It aims at establishing conditions for the cryopreservation of cultured neuronal cells readily usable, upon thawing. That is cryopreserved neuronal cells with a high rate of survivability and with a potential to differentiate into mature neuronal cells rapidly, after thawing. METHODS: Neuronal precursor cells and neuronal cells were generated in vitro, from cultures of human ESCs (hESCs). Neuronal cells were frozen and cryopreserved at a stage when they begin to express neuronal class III beta-tubulin. RESULTS/ CONCLUSION: The application claims that neuronal cells elicit a rate of survivability greater than 50% and neurites outgrowth within 10 to 14 hours, after thawing. The cryopreservation of post-mitotic neuronal or neural-like cells provides a source of nerve cells readily usable for research and therapy.
BACKGROUND: The application is in the field of embryonic stem cells (ESCs) and the cryopreservation of neuronal cells in culture. OBJECTIVE: It aims at establishing conditions for the cryopreservation of cultured neuronal cells readily usable, upon thawing. That is cryopreserved neuronal cells with a high rate of survivability and with a potential to differentiate into mature neuronal cells rapidly, after thawing. METHODS: Neuronal precursor cells and neuronal cells were generated in vitro, from cultures of human ESCs (hESCs). Neuronal cells were frozen and cryopreserved at a stage when they begin to express neuronal class III beta-tubulin. RESULTS/ CONCLUSION: The application claims that neuronal cells elicit a rate of survivability greater than 50% and neurites outgrowth within 10 to 14 hours, after thawing. The cryopreservation of post-mitotic neuronal or neural-like cells provides a source of nerve cells readily usable for research and therapy.