| Literature DB >> 19439447 |
Masaki Mori1, Hironori Nakagami, Nobutaka Koibuchi, Koichi Miura, Yoichi Takami, Hiroshi Koriyama, Hiroki Hayashi, Hisataka Sabe, Naoki Mochizuki, Ryuichi Morishita, Yasufumi Kaneda.
Abstract
Epithelial-mesenchymal transition (EMT) confers destabilization of cell-cell adhesion and cell motility required for morphogenesis or cancer metastasis. Here we report that zyxin, a focal adhesion-associated LIM protein, is essential for actin reorganization for cell migration in TGF-beta1-induced EMT in normal murine mammary gland (NMuMG) cells. TGF-beta1 induced the relocation of zyxin from focal adhesions to actin fibers. In addition, TGF-beta1 up-regulated zyxin via a transcription factor, Twist1. Depletion of either zyxin or Twist1 abrogated the TGF-beta1-dependent EMT, including enhanced cell motility and actin reorganization, indicating the TGF-beta1-Twist1-zyxin signal for EMT. Both zyxin and Twist1 were predominantly expressed in the cardiac atrioventricular canal (AVC) that undergoes EMT during heart development. We further performed ex vivo AVC explant assay and revealed that zyxin was required for the reorganization of actin fibers and migration of the endocardial cells. Thus, zyxin reorganizes actin fibers and enhances cell motility in response to TGF-beta1, thereby regulating EMT.Entities:
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Year: 2009 PMID： 19439447 PMCID： PMC2704162 DOI： 10.1091/mbc.e09-01-0046
Source DB: PubMed Journal: Mol Biol Cell ISSN： 1059-1524 Impact factor: 4.138