E Varga1, M Kiss, K Szabó, L Kemény. 1. Department of Dermatology and Allergology, University of Szeged, H-6720 Szeged, Hungary. vargerik@mail.derma.szote.u-szeged.hu
Abstract
BACKGROUND: Merkel cell carcinoma (MCC) is a rare, aggressive tumour for which an increasing incidence has been reported. A new human polyomavirus, Merkel cell polyomavirus (MCV), was recently isolated from these tumours by applying digital transcriptome subtraction methodology. OBJECTIVES: To detect the presence or absence of MCV in MCCs and other, randomly selected neoplasms. METHODS: Nine primary or recurrent MCCs from seven patients were examined; 29 other tumours (squamous cell, basal cell and basosquamous carcinomas and malignant melanomas) were examined for comparative purposes. Viral large T protein (LT1 and LT3), and viral capsid protein (VP1) were detected by primer-directed amplification, using a polymerase chain reaction (PCR)-based method, and the amplified PCR products were analysed by agarose gel electrophoresis and subsequent sequence analysis. RESULTS: The presence of viral T antigen and/or viral capsid DNA sequences was demonstrated in seven of the eight MCC lesions. None of the comparative samples contained MCV DNA. CONCLUSIONS: Our findings strongly support the hypothesis that MCV infection may well be specific for MCC, and MCV may play a role in the pathogenesis of MCC.
BACKGROUND:Merkel cell carcinoma (MCC) is a rare, aggressive tumour for which an increasing incidence has been reported. A new humanpolyomavirus, Merkel cell polyomavirus (MCV), was recently isolated from these tumours by applying digital transcriptome subtraction methodology. OBJECTIVES: To detect the presence or absence of MCV in MCCs and other, randomly selected neoplasms. METHODS: Nine primary or recurrent MCCs from seven patients were examined; 29 other tumours (squamous cell, basal cell and basosquamous carcinomas and malignant melanomas) were examined for comparative purposes. Viral large T protein (LT1 and LT3), and viral capsid protein (VP1) were detected by primer-directed amplification, using a polymerase chain reaction (PCR)-based method, and the amplified PCR products were analysed by agarose gel electrophoresis and subsequent sequence analysis. RESULTS: The presence of viral T antigen and/or viral capsid DNA sequences was demonstrated in seven of the eight MCC lesions. None of the comparative samples contained MCV DNA. CONCLUSIONS: Our findings strongly support the hypothesis that MCV infection may well be specific for MCC, and MCV may play a role in the pathogenesis of MCC.
Authors: Dana E Rollison; Anna R Giuliano; Jane L Messina; Neil A Fenske; Basil S Cherpelis; Vernon K Sondak; Richard G Roetzheim; Michelle R Iannacone; Kristina M Michael; Tarik Gheit; Tim Waterboer; Massimo Tommasino; Michael Pawlita Journal: Cancer Epidemiol Biomarkers Prev Date: 2011-10-20 Impact factor: 4.254
Authors: Linlin Li; Joseph Victoria; Amit Kapoor; Olga Blinkova; Chunlin Wang; Farbod Babrzadeh; Carl J Mason; Prativa Pandey; Hinda Triki; Olfa Bahri; Bamidele Soji Oderinde; Marycelin Mandu Baba; David Nadeba Bukbuk; John M Besser; Joanne M Bartkus; Eric L Delwart Journal: J Virol Date: 2009-09-16 Impact factor: 5.103
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