Maike Keck1, Juergen Janke, Klaus Ueberreiter. 1. Department of Plastic and Aesthetic Surgery, Asklepios Clinic Birkenwerder, Birkenwerder, Germany. maike.keck@meduniwien.ac.at
Abstract
BACKGROUND: Autogenous fat transfer with lipoinjection for soft tissue augmentation is a commonly used surgical technique. Abundant donor tissue availability and relative ease of harvesting have made autologous fat an attractive soft tissue filler. The overall reliability of this technique is often disputed, and different authors describe different results after autologous fat transplantation despite using similar techniques. In this study, we examined the influence of different local anesthetics commonly used in fat harvest and the pH of the anesthetic solution on the viability of harvested preadipocytes. METHODS AND MATERIALS: Preadipocytes were incubated with 1% lidocaine, 1% articaine plus epinephrine 1:200,000, 0.75% ropivacaine, and 1% prilocaine or our standardized tumescent solution (1 L of 0.9% sodium chloride solution plus 25 mL of 1% articaine plus epinephrine 1:200,000 plus 25 mL of bicarbonate) for 30 minutes. Additionally, we incubated cells with the local anesthetics as described above but diluted 1:2 with phosphate buffered saline (pH 7.4). Viability was measured using trypan blue dying as well as propidium iodine staining and fluorescence-activated cell sorting analysis. RESULTS: There are significant differences in the viability of preadipocytes under the influence of various local anesthetics. DISCUSSION: Our data could partially explain the varying results after autogenous fat transfer.
BACKGROUND: Autogenous fat transfer with lipoinjection for soft tissue augmentation is a commonly used surgical technique. Abundant donor tissue availability and relative ease of harvesting have made autologous fat an attractive soft tissue filler. The overall reliability of this technique is often disputed, and different authors describe different results after autologous fat transplantation despite using similar techniques. In this study, we examined the influence of different local anesthetics commonly used in fat harvest and the pH of the anesthetic solution on the viability of harvested preadipocytes. METHODS AND MATERIALS: Preadipocytes were incubated with 1% lidocaine, 1% articaine plus epinephrine 1:200,000, 0.75% ropivacaine, and 1% prilocaine or our standardized tumescent solution (1 L of 0.9% sodium chloride solution plus 25 mL of 1% articaine plus epinephrine 1:200,000 plus 25 mL of bicarbonate) for 30 minutes. Additionally, we incubated cells with the local anesthetics as described above but diluted 1:2 with phosphate buffered saline (pH 7.4). Viability was measured using trypan blue dying as well as propidium iodine staining and fluorescence-activated cell sorting analysis. RESULTS: There are significant differences in the viability of preadipocytes under the influence of various local anesthetics. DISCUSSION: Our data could partially explain the varying results after autogenous fat transfer.
Authors: Maurizio B Nava; Phillip Blondeel; Giovanni Botti; Francesco Casabona; Giuseppe Catanuto; Mark W Clemens; Domenico De Fazio; Roy De Vita; James Grotting; Dennis C Hammond; Paul Harris; Paolo Montemurro; Alexandre Mendonça Munhoz; Maurice Nahabedian; Stefano Pompei; Alberto Rancati; Gino Rigotti; Marzia Salgarello; Gianfranco Scaperrotta; Andrea Spano; Costantin Stan; Nicola Rocco Journal: Plast Reconstr Surg Glob Open Date: 2019-10-28
Authors: Young Hoon Kim; Ga Young Park; Nechama Rabinovitch; Solaiman Tarafder; Chang H Lee Journal: Stem Cell Res Ther Date: 2020-09-07 Impact factor: 6.832