BACKGROUND: Variations occur in the pH of cutaneous wounds which may affect wound closure, graft take and microbial infection rates. OBJECTIVES: To determine how pH modulates cell behaviour in order to optimize wound care. METHODS: The effects of pH on the attachment, proliferation and migration of keratinocytes and fibroblasts were investigated in vitro and in an ex vivo skin growth model. In addition, the effect of pH on keratinocyte differentiation as measured by the expression of cytokeratins 1 (K1) and K5 was studied. RESULTS: We demonstrated that the optimal pH for both keratinocyte and fibroblast proliferation is between pH 7.2 and 8.3. The optimal pH for growth from ex vivo skin explants was pH 8.43 which correlates with a previously reported improvement in skin graft take at higher pHs. Expression of K1 was found to be elevated in keratinocytes at a low pH. CONCLUSIONS: These results demonstrate that skin cells and explants proliferate and migrate at pHs higher than the physiological pH and that at lower pH keratinocytes express a differentiated keratinocyte phenotype. A better understanding of the responses of the cellular components of skin to fundamental physiological variables such as pH may help inform improved clinical wound care.
BACKGROUND: Variations occur in the pH of cutaneous wounds which may affect wound closure, graft take and microbial infection rates. OBJECTIVES: To determine how pH modulates cell behaviour in order to optimize wound care. METHODS: The effects of pH on the attachment, proliferation and migration of keratinocytes and fibroblasts were investigated in vitro and in an ex vivo skin growth model. In addition, the effect of pH on keratinocyte differentiation as measured by the expression of cytokeratins 1 (K1) and K5 was studied. RESULTS: We demonstrated that the optimal pH for both keratinocyte and fibroblast proliferation is between pH 7.2 and 8.3. The optimal pH for growth from ex vivo skin explants was pH 8.43 which correlates with a previously reported improvement in skin graft take at higher pHs. Expression of K1 was found to be elevated in keratinocytes at a low pH. CONCLUSIONS: These results demonstrate that skin cells and explants proliferate and migrate at pHs higher than the physiological pH and that at lower pH keratinocytes express a differentiated keratinocyte phenotype. A better understanding of the responses of the cellular components of skin to fundamental physiological variables such as pH may help inform improved clinical wound care.
Authors: S L Rajasekhar Karna; Peter D'Arpa; Tsute Chen; Li-Wu Qian; Andrea B Fourcaudot; Kazuyoshi Yamane; Ping Chen; Johnathan J Abercrombie; Tao You; Kai P Leung Journal: PLoS One Date: 2016-10-28 Impact factor: 3.240
Authors: Arianna Pompilio; Giuseppe Galardi; Fabio Verginelli; Maurizio Muzzi; Andrea Di Giulio; Giovanni Di Bonaventura Journal: Front Microbiol Date: 2017-12-22 Impact factor: 5.640
Authors: W Nathaniel Brennen; Daniel L J Thorek; Wen Jiang; Timothy E Krueger; Lizamma Antony; Samuel R Denmeade; John T Isaacs Journal: Immunotherapy Date: 2020-11-05 Impact factor: 4.196