Feng Ji1, Xi Jin, Chun-Hua Jiao, Qin-Wei Xu, Zi-Wei Wang, Yue-Liang Chen. 1. Department of Digestive Diseases, First Affiliated Hospital of Zhejiang University Medical School, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jifeng1126@sina.com
Abstract
AIM: To investigate the role of FAT10 and mutant p53 in the pathogenesis, severity and prognosis of gastric cancer. METHODS: FAT10, mutant p53 mRNA and protein levels were measured by reverse transcription (RT)-PCR and immunohistochemistry in gastric cancer tissue (n = 62), tumor-adjacent tissue (n = 62) and normal gastric tissue (n = 62). Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancer patients were analyzed. RESULTS: The FAT10, mutant p53 mRNA and protein levels were significantly higher in gastric cancer than in its adjacent and normal tissue. The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor, nodes, metastasis (TNM) staging. Moreover, the high FAT10 level was associated with the overall survival rate of patients. Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53, lymph node metastasis, distant metastasis and TNM stage were the independent prognostic factors for gastric cancer. CONCLUSION: FAT10 may be involved in gastric carcinogenesis, and is a potential marker for the prognosis of gastric cancer patients. FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.
AIM: To investigate the role of FAT10 and mutant p53 in the pathogenesis, severity and prognosis of gastric cancer. METHODS: FAT10, mutant p53 mRNA and protein levels were measured by reverse transcription (RT)-PCR and immunohistochemistry in gastric cancer tissue (n = 62), tumor-adjacent tissue (n = 62) and normal gastric tissue (n = 62). Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancerpatients were analyzed. RESULTS: The FAT10, mutant p53 mRNA and protein levels were significantly higher in gastric cancer than in its adjacent and normal tissue. The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor, nodes, metastasis (TNM) staging. Moreover, the high FAT10 level was associated with the overall survival rate of patients. Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53, lymph node metastasis, distant metastasis and TNM stage were the independent prognostic factors for gastric cancer. CONCLUSION: FAT10 may be involved in gastric carcinogenesis, and is a potential marker for the prognosis of gastric cancerpatients. FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.
Authors: M Rugge; Y H Shiao; G Busatto; M Cassaro; C Strobbe; V M Russo; G Leo; A R Parenti; A Scapinello; P Arslan; E Egarter-Vigl Journal: Mol Pathol Date: 2000-08
Authors: Caroline G L Lee; Jianwei Ren; Ian S Y Cheong; Kenneth H K Ban; London L P J Ooi; Soo Yong Tan; Alison Kan; Issarang Nuchprayoon; Rongxian Jin; Kang-Hoe Lee; Michael Choti; Linda A Lee Journal: Oncogene Date: 2003-05-01 Impact factor: 9.867