Literature DB >> 19430544

Investigation of molecular recognition in biological systems using cellular membrane affinity chromatography.

Irving W Wainer.   

Abstract

Cellular membrane affinity chromatography (CMAC) columns have been created through the immobilization of cellular membrane fragments on liquid chromatographic supports. A CMAC column containing the human organic cation transporter, CMAC(hOCT1) column, has been used to study the stereoselective binding of competitive inhibitors. The chromatographic data obtained using the CMAC(hOCT1) column was to develop a pharmacophore model that described the stereoselectivity. The results indicate that a dynamic chiral recognition model based upon conformational adjustments between the inhibitors and hOCT1 is responsible for the observed steroeselectivity.

Entities:  

Year:  2008        PMID： 19430544      PMCID： PMC2678806     

Source DB:  PubMed          Journal:  Chim Oggi        ISSN： 0392-839X


  19 in total

1.  Multiple receptor liquid chromatographic stationary phases: the co-immobilization of nicotinic receptors, gamma-amino-butyric acid receptors, and N-methyl D-aspartate receptors.

Authors:  Ruin Moaddel; Jean-François Cloix; Gözen Ertem; Irving W Wainer
Journal:  Pharm Res       Date:  2002-01       Impact factor: 4.200

2.  Syntheses of immobilized G protein-coupled receptor chromatographic stationary phases: characterization of immobilized mu and kappa opioid receptors.

Authors:  Farideh Beigi; Irving W Wainer
Journal:  Anal Chem       Date:  2003-09-01       Impact factor: 6.986

Review 3.  Allosteric modifiers of neuronal nicotinic acetylcholine receptors: new methods, new opportunities.

Authors:  Ruin Moaddel; Krzysztof Jozwiak; Irving W Wainer
Journal:  Med Res Rev       Date:  2007-09       Impact factor: 12.944

4.  Interaction of noncompetitive inhibitors with the alpha3beta2 nicotinic acetylcholine receptor investigated by affinity chromatography and molecular docking.

Authors:  Krzysztof Jozwiak; Sarangan Ravichandran; Jack R Collins; Ruin Moaddel; Irving W Wainer
Journal:  J Med Chem       Date:  2007-10-31       Impact factor: 7.446

5.  The synthesis and initial characterization of an immobilized purinergic receptor (P2Y1) liquid chromatography stationary phase for online screening.

Authors:  Ruin Moaddel; Enrica Calleri; Gabriella Massolini; Chester R Frazier; Irving W Wainer
Journal:  Anal Biochem       Date:  2007-02-22       Impact factor: 3.365

6.  Screening of tobacco smoke condensate for nicotinic acetylcholine receptor ligands using cellular membrane affinity chromatography columns and missing peak chromatography.

Authors:  Alexandre Maciuk; Ruin Moaddel; Jun Haginaka; Irving W Wainer
Journal:  J Pharm Biomed Anal       Date:  2007-11-26       Impact factor: 3.935

7.  G-protein-coupled receptor chromatographic stationary phases. 2. Ligand-induced conformational mobility in an immobilized beta2-adrenergic receptor.

Authors:  Farideh Beigi; Khalid Chakir; Rui-Ping Xiao; Irving W Wainer
Journal:  Anal Chem       Date:  2004-12-15       Impact factor: 6.986

8.  Pharmacophore modelling of stereoselective binding to the human organic cation transporter (hOCT1).

Authors:  R Moaddel; S Ravichandran; F Bighi; R Yamaguchi; I W Wainer
Journal:  Br J Pharmacol       Date:  2007-06-25       Impact factor: 8.739

9.  Identification of P-glycoprotein substrates using open tubular chromatography on an immobilized P-glycoprotein column: Comparison of chromatographic results with Caco-2 permeability.

Authors:  Ruin Moaddel; Rachid Hamid; Sharvil Patel; Peter L Bullock; Irving W Wainer
Journal:  Anal Chim Acta       Date:  2006-03-10       Impact factor: 6.558

10.  Exploring enantiospecific ligand-protein interactions using cellular membrane affinity chromatography: chiral recognition as a dynamic process.

Authors:  Krzysztof Jozwiak; Ruin Moaddel; Sarangan Ravichandran; Anita Plazinska; Joanna Kozak; Sharvil Patel; Rika Yamaguchi; Irving W Wainer
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-11-01       Impact factor: 3.205
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