| Literature DB >> 19428591 |
Anne Böhmer1, Verena Schildgen, Jessica Lüsebrink, Susanne Ziegler, Ramona L Tillmann, Michael Kleines, Oliver Schildgen.
Abstract
Human bocavirus (HBoV), solely based on phylogenetic analyses, was classified as the second autonomous human parvovirus. Unfortunately, neither susceptible cell cultures nor animal models were described hitherto, thus complicating studies on viral genome structure and replication steps. A novel application of nucleic acid sequence-based amplification (NASBA) revealed that in all tested samples (100%) that became positive by NASBA the negative strand of the HBoV genome was packaged. Additionally, two of those samples also contained a detectable amount of positive strand (14.3%). The data confirm the assumed single-stranded negative-sense nature of HBoV-genomes that is independent of the viral subtype while showing that NASBA is useful not only for diagnosis.Entities:
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Year: 2009 PMID： 19428591 DOI： 10.1016/j.jviromet.2009.02.010
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014