| Literature DB >> 19425501 |
T V Pavlova, V I Kashuba, O V Muravenko, S P Yenamandra, T A Ivanova, V I Zabarovskaia, E R Rakhmanaliev, L A Petrenko, I V Pronina, V I Loginov, O Iu Iurkevich, L L Kiselev, A V Zelenin, E R Zabarovskiĭ.
Abstract
New comparative genome hybridization technology on NotI-microarrays is presented (Karolinska Institute International Patent WO02/086163). The method is based on comparative genome hybridization of NotI-probes from tumor and normal genomic DNA with the principle of new DNA NotI-microarrays. Using this method 181 NotI linking loci from human chromosome 3 were analyzed in 200 malignant tumor samples from different organs: kidney, lung, breast, ovary, cervical, prostate. Most frequently (more than in 30%) aberrations--deletions, methylation,--were identified in NotI-sites located in MINT24, BHLHB2, RPL15, RARbeta1, ITGA9, RBSP3, VHL, ZIC4 genes, that suggests they probably are involved in cancer development. Methylation of these genomic loci was confirmed by methylation-specific PCR and bisulfite sequencing. The results demonstrate perspective of using this method to solve some oncogenomic problems.Entities:
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Year: 2009 PMID： 19425501
Source DB: PubMed Journal: Mol Biol (Mosk) ISSN： 0026-8984