OBJECTIVE: To investigate the accumulation of advanced glycation end products (AGE) in human bladder. METHODS: Human bladder specimens were obtained from nine patients during radical cystectomy. Frozen sections were immunohistochemically analyzed by three different monoclonal anti-AGE antibodies such as anti-N(epsilon)-(carboxymethyl)lysine (CML), anti-imidazolone and anti-pentosidine antibodies. Bladder sections were stained with these antibodies by indirect immunoperoxidase methods. Double immunohistochemical staining with one of the anti-AGE antibodies or an anti-human macrophage antibody was also carried out. RESULTS: We demonstrated that CML and pentosidine were accumulated in human bladder extracellularly as well as intracellularly, whereas any accumulation of imidazolone was not observed. Double immunohistochemical staining indicated that AGE-accumulated cells in human bladder were derived from macrophages. CONCLUSIONS: The present study demonstrated that AGE-structures such as CML and pentosidine are accumulated extracellularly in human bladder, and were endocytosed by tissue macrophages.
OBJECTIVE: To investigate the accumulation of advanced glycation end products (AGE) in human bladder. METHODS:Human bladder specimens were obtained from nine patients during radical cystectomy. Frozen sections were immunohistochemically analyzed by three different monoclonal anti-AGE antibodies such as anti-N(epsilon)-(carboxymethyl)lysine (CML), anti-imidazolone and anti-pentosidine antibodies. Bladder sections were stained with these antibodies by indirect immunoperoxidase methods. Double immunohistochemical staining with one of the anti-AGE antibodies or an anti-human macrophage antibody was also carried out. RESULTS: We demonstrated that CML and pentosidine were accumulated in human bladder extracellularly as well as intracellularly, whereas any accumulation of imidazolone was not observed. Double immunohistochemical staining indicated that AGE-accumulated cells in human bladder were derived from macrophages. CONCLUSIONS: The present study demonstrated that AGE-structures such as CML and pentosidine are accumulated extracellularly in human bladder, and were endocytosed by tissue macrophages.
Authors: Sheldon Rowan; Shuhong Jiang; Tal Korem; Jedrzej Szymanski; Min-Lee Chang; Jason Szelog; Christa Cassalman; Kalavathi Dasuri; Christina McGuire; Ryoji Nagai; Xue-Liang Du; Michael Brownlee; Naila Rabbani; Paul J Thornalley; James D Baleja; Amy A Deik; Kerry A Pierce; Justin M Scott; Clary B Clish; Donald E Smith; Adina Weinberger; Tali Avnit-Sagi; Maya Lotan-Pompan; Eran Segal; Allen Taylor Journal: Proc Natl Acad Sci U S A Date: 2017-05-15 Impact factor: 11.205