Literature DB >> 194234

Purification of thioredoxin reductase from the Novikoff rat tumor.

C C Chen, B L McCall, E C Moore.   

Abstract

Thioredoxin reductase (E.C.1.6.4.5.) has been purified to about 95% homogeneity from the Novikoff ascites rat tumor. The enzyme contained two subunits of approximately 58,000 daltons, with one FAD per subunit. The amino acid analysis is reported. An immunoadsorbent was prepared and used for affinity chromatography in order to improve the yield of the enzyme.

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Year:  1977        PMID： 194234     DOI： 10.1080/00327487708061633

Source DB:  PubMed          Journal:  Prep Biochem        ISSN： 0032-7484


  7 in total

Review 1.  Thioredoxin reductase.

Authors:  D Mustacich; G Powis
Journal:  Biochem J       Date:  2000-02-15       Impact factor: 3.857

2.  Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene.

Authors:  V N Gladyshev; K T Jeang; T C Stadtman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

3.  Heparin-binding properties of selenium-containing thioredoxin reductase from HeLa cells and human lung adenocarcinoma cells.

Authors:  S Y Liu; T C Stadtman
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

4.  Thioredoxin/Glutaredoxin System of Chlorella: CHLORELLA ADENOSINE 5'-PHOSPHOSULFATE SULFOTRANSFERASE CANNOT USE THIOREDOXIN OR GLUTAREDOXIN AS COFACTORS.

Authors:  M L Tsang
Journal:  Plant Physiol       Date:  1981-11       Impact factor: 8.340

5.  Thioredoxin, glutaredoxin, and thioredoxin reductase from cultured HeLa cells.

Authors:  M L Tsang; J A Weatherbee
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

6.  Glutathione-dependent hydrogen donor system for calf thymus ribonucleoside-diphosphate reductase.

Authors:  M Luthman; S Eriksson; A Holmgren; L Thelander
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

7.  A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity.

Authors:  T Tamura; T C Stadtman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-06       Impact factor: 11.205
  7 in total

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