OBJECTIVE: To develop and test a novel approach to xenotransplantation of isolated preantral follicles underneath the kidney capsule of immunodeficient mice. DESIGN: Prospective experimental animal study. SETTING: Academic research unit. ANIMAL(S): Healthy adult nude mice. INTERVENTION(S): Bovine ovaries from fetuses (n = 3) and calves (n = 3) were enzymatically disaggregated and subsequently filtered. Isolated preantral follicles were suspended in phosphate buffered saline, and granulosa and stroma cells originating from the ovarian digest served as embedding matrix. The suspension was injected under the kidney capsule of adult nude mice. MAIN OUTCOME MEASURE(S): Fourteen days after transplantation, follicular survival and proliferation in grafts was assessed by histology and proliferating cell nuclear antigen (PCNA) immunostaining, and was compared with ungrafted control tissue. RESULT(S): Primordial follicles decreased from 58.2% in control tissue to 17.1% in transplants in the fetal group, and from 76.0% to 17.2% in the calf group. Concomitantly, primary follicles increased from 13.4% to 62.2% in the fetal group, and from 5.4% to 63.5% in the calf group. Follicular proliferation measured by PCNA immunolabeling exhibited an increase from 40.6% growing follicles to 81.9% in the fetal group, and from 21.0% to 80.7% in the calf group. CONCLUSION(S): The massive follicular activation following transplantation indicates that isolated preantral follicles are able to survive and grow 14 days after renal subcapsular xenotransplantation. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To develop and test a novel approach to xenotransplantation of isolated preantral follicles underneath the kidney capsule of immunodeficientmice. DESIGN: Prospective experimental animal study. SETTING: Academic research unit. ANIMAL(S): Healthy adult nude mice. INTERVENTION(S): Bovine ovaries from fetuses (n = 3) and calves (n = 3) were enzymatically disaggregated and subsequently filtered. Isolated preantral follicles were suspended in phosphate buffered saline, and granulosa and stroma cells originating from the ovarian digest served as embedding matrix. The suspension was injected under the kidney capsule of adult nude mice. MAIN OUTCOME MEASURE(S): Fourteen days after transplantation, follicular survival and proliferation in grafts was assessed by histology and proliferating cell nuclear antigen (PCNA) immunostaining, and was compared with ungrafted control tissue. RESULT(S): Primordial follicles decreased from 58.2% in control tissue to 17.1% in transplants in the fetal group, and from 76.0% to 17.2% in the calf group. Concomitantly, primary follicles increased from 13.4% to 62.2% in the fetal group, and from 5.4% to 63.5% in the calf group. Follicular proliferation measured by PCNA immunolabeling exhibited an increase from 40.6% growing follicles to 81.9% in the fetal group, and from 21.0% to 80.7% in the calf group. CONCLUSION(S): The massive follicular activation following transplantation indicates that isolated preantral follicles are able to survive and grow 14 days after renal subcapsular xenotransplantation. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Anniek Bus; Veerle van Hoeck; An Langbeen; Jo L M R Leroy; Peter E J Bols Journal: J Assist Reprod Genet Date: 2018-05-24 Impact factor: 3.412
Authors: Luciana L Damous; Juliana S Nakamuta; Ana E T Saturi de Carvalho; Katia Candido Carvalho; José Maria Soares; Manuel de Jesus Simões; José Eduardo Krieger; Edmund Chada Baracat Journal: Reprod Biol Endocrinol Date: 2015-09-23 Impact factor: 5.211
Authors: Luciana Lamarão Damous; Juliana Sanajotti Nakamuta; José Maria Soares; Gustavo Arantes Rosa Maciel; Ricardo Dos Santos Simões; Edna Frasson de Souza Montero; José Eduardo Krieger; Edmund Chada Baracat Journal: J Ovarian Res Date: 2014-03-20 Impact factor: 4.234