INTRODUCTION: There are circadian genes whose expressions were affected by sevoflurane. We hypothesized that propofol and dexmedetomidine also have influences on the expressions of circadian genes. MATERIALS AND METHODS: Seven-week-old rats were cannulated and propofol (600 microg/kg/min, 1 ml/h, n=6: group P6H), dexmedetomidine (1 microg/kg/min, 1 ml/h, n=6: group D6H), 10% lipid (1 ml/h, n=6: group L) and normal saline (1 ml/h, n=6: group N) were infused intravenously for 6h; rats were decapitated soon or 24h after awakening from anesthesia (groups P24H n=6 and D24H n=6). Real time reverse transcription-polymerase chain reaction was performed using rat whole brain and the expressions of circadian genes were measured. RESULTS: In the P6H group and the P24H group, the whole expressions of seven genes were changed significantly compared with the L group. In the D6H group, the whole expression of seven genes was changed significantly compared with the N group. In the P6H group, all gene expressions except for Tef (thyrotroph embryonic factor) were changed significantly compared with the L group. In this group, the expression of Dbp (D site albumin promoter binding protein) was increased, and the others were decreased. In the D6H group, all gene expressions except for Dbp and Tef were decreased significantly compared with the N group. For the two anesthetics, the expression patterns were different only in Dbp. CONCLUSION: We showed that propofol and dexmedetomidine have influences on the circadian gene expressions.
INTRODUCTION: There are circadian genes whose expressions were affected by sevoflurane. We hypothesized that propofol and dexmedetomidine also have influences on the expressions of circadian genes. MATERIALS AND METHODS: Seven-week-old rats were cannulated and propofol (600 microg/kg/min, 1 ml/h, n=6: group P6H), dexmedetomidine (1 microg/kg/min, 1 ml/h, n=6: group D6H), 10% lipid (1 ml/h, n=6: group L) and normal saline (1 ml/h, n=6: group N) were infused intravenously for 6h; rats were decapitated soon or 24h after awakening from anesthesia (groups P24H n=6 and D24H n=6). Real time reverse transcription-polymerase chain reaction was performed using rat whole brain and the expressions of circadian genes were measured. RESULTS: In the P6H group and the P24H group, the whole expressions of seven genes were changed significantly compared with the L group. In the D6H group, the whole expression of seven genes was changed significantly compared with the N group. In the P6H group, all gene expressions except for Tef (thyrotroph embryonic factor) were changed significantly compared with the L group. In this group, the expression of Dbp (D site albumin promoter binding protein) was increased, and the others were decreased. In the D6H group, all gene expressions except for Dbp and Tef were decreased significantly compared with the N group. For the two anesthetics, the expression patterns were different only in Dbp. CONCLUSION: We showed that propofol and dexmedetomidine have influences on the circadian gene expressions.
Authors: Necati Gökmen; İbrahim Barış; Elvan Öçmen; Osman Yılmaz; Ali Günerli; İbrahim Halil Kavaklı Journal: Turk J Anaesthesiol Reanim Date: 2017-08-01
Authors: Alexander S Mathew; Catherine M Gorick; E Andrew Thim; William J Garrison; Alexander L Klibanov; G Wilson Miller; Natasha D Sheybani; Richard J Price Journal: Bioeng Transl Med Date: 2020-11-24