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Literature DB >> 194225

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

R Contreras, G Volckaert, F Thys, A Van de Voorde, W Fiers.

Abstract

The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.

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Year: 1977 PMID： 194225 PMCID： PMC342500 DOI： 10.1093/nar/4.4.1001

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

1. Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.

2. Sequences spanning the EcoRI substrate site.

3. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.

4. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.

5. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

6. Capsid proteins of Simian virus 40.

7. Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron.

8. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

9. Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemopholus aegyptius.

10. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system.

1. Substrate dependence of the mechanism of EcoRI endonuclease.

2. Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

3. Nucleotide sequence of the gene for the major structural protein of SV40 virus.