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Literature DB >> 19421423

NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA.

Geoffrey A Mueller¹, Eugene F DeRose, Thomas W Kirby, Robert E London.

Abstract

DNA polymerase beta is a multifunctional enzyme involved in base excision repair of nuclear DNA invertebrate cells. We present here the first assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa).

Entities: Chemical Gene

Keywords: Base Excision Repair; Polymeraseβ

Mesh：

Substances：

Year: 2007 PMID： 19421423 PMCID： PMC2676717 DOI： 10.1007/s12104-007-9007-2

Source DB: PubMed Journal: Biomol NMR Assign ISSN： 1874-270X Impact factor: 0.746

11 in total

1. PACES: Protein sequential assignment by computer-assisted exhaustive search.

Authors: Brian E Coggins; Pei Zhou
Journal: J Biomol NMR Date: 2003-06 Impact factor: 2.835

2. MONTE: An automated Monte Carlo based approach to nuclear magnetic resonance assignment of proteins.

Authors: T Kevin Hitchens; Jonathan A Lukin; Yiping Zhan; Scott A McCallum; Gordon S Rule
Journal: J Biomol NMR Date: 2003-01 Impact factor: 2.835

3. NvAssign: protein NMR spectral assignment with NMRView.

Authors: Nigel I Kirby; Eugene F DeRose; Robert E London; Geoffrey A Mueller
Journal: Bioinformatics Date: 2004-02-10 Impact factor: 6.937

4. Increased sensitivity in HNCA and HN(CO)CA experiments by selective C beta decoupling.

Authors: H Matsuo; E Kupce; H Li; G Wagner
Journal: J Magn Reson B Date: 1996-10

5. 1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase beta.

Authors: Michael R Gryk; Mark W Maciejewski; Anthony Robertson; Mary A Mullen; Samuel H Wilson; Gregory P Mullen
Journal: J Biomol NMR Date: 2002-02 Impact factor: 2.835

6. Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

Authors: D Marion; P C Driscoll; L E Kay; P T Wingfield; A Bax; A M Gronenborn; G M Clore
Journal: Biochemistry Date: 1989-07-25 Impact factor: 3.162

7. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors: K Pervushin; R Riek; G Wider; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 1997-11-11 Impact factor: 11.205

8. Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta.

Authors: Bidisha Bose-Basu; Eugene F DeRose; Thomas W Kirby; Geoffrey A Mueller; William A Beard; Samuel H Wilson; Robert E London
Journal: Biochemistry Date: 2004-07-20 Impact factor: 3.162

9. Assignments of 1H, 15N, and 13C resonances for the backbone and side chains of the N-terminal domain of DNA polymerase beta. Determination of the secondary structure and tertiary contacts.

Authors: D Liu; E F DeRose; R Prasad; S H Wilson; G P Mullen
Journal: Biochemistry Date: 1994-08-16 Impact factor: 3.162

10. A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors: M Ikura; L E Kay; A Bax
Journal: Biochemistry Date: 1990-05-15 Impact factor: 3.162

5 in total

NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA.

1. PACES: Protein sequential assignment by computer-assisted exhaustive search.

2. MONTE: An automated Monte Carlo based approach to nuclear magnetic resonance assignment of proteins.

3. NvAssign: protein NMR spectral assignment with NMRView.

4. Increased sensitivity in HNCA and HN(CO)CA experiments by selective C beta decoupling.

5. 1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase beta.

6. Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

7. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

8. Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta.

9. Assignments of 1H, 15N, and 13C resonances for the backbone and side chains of the N-terminal domain of DNA polymerase beta. Determination of the secondary structure and tertiary contacts.

10. A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

1. Substrate-dependent millisecond domain motions in DNA polymerase β.

2. Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis.

3. The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins.

4. Metal-induced DNA translocation leads to DNA polymerase conformational activation.

5. Substrate rescue of DNA polymerase β containing a catastrophic L22P mutation.