Literature DB >> 19420695

Development of a homologous transformation system for Aspergillus aculeatus based on the sC gene encoding ATP-sulfurylase.

Hiromi Adachi1, Shuji Tani, Shin Kanamasa, Jun-ichi Sumitani, Takashi Kawaguchi.   

Abstract

A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC(+) transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.

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Year:  2009        PMID： 19420695     DOI： 10.1271/bbb.80772

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  5 in total

1.  A component of the septation initiation network complex, AaSepM, is involved in multiple cellulose-responsive signaling pathways in Aspergillus aculeatus.

Authors:  Ryosuke Tsumura; Kazumi Sawada; Emi Kunitake; Jun-Ichi Sumitani; Takashi Kawaguchi; Shuji Tani
Journal:  Appl Microbiol Biotechnol       Date:  2021-01-22       Impact factor: 4.813

2.  XlnR-independent signaling pathway regulates both cellulase and xylanase genes in response to cellobiose in Aspergillus aculeatus.

Authors:  Shuji Tani; Shin Kanamasa; Jun-ichi Sumitani; Motoo Arai; Takashi Kawaguchi
Journal:  Curr Genet       Date:  2012-02-28       Impact factor: 3.886

3.  Serine-arginine protein kinase-like protein, SrpkF, stimulates both cellobiose-responsive and D-xylose-responsive signaling pathways in Aspergillus aculeatus.

Authors:  Ryohei Katayama; Natsumi Kobayashi; Takashi Kawaguchi; Shuji Tani
Journal:  Curr Genet       Date:  2021-08-28       Impact factor: 3.886

4.  Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis.

Authors:  Emi Kunitake; Shuji Tani; Jun-Ichi Sumitani; Takashi Kawaguchi
Journal:  AMB Express       Date:  2011-12-14       Impact factor: 3.298

5.  Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus.

Authors:  Shuji Tani; Atsushi Tsuji; Emi Kunitake; Jun-Ichi Sumitani; Takashi Kawaguchi
Journal:  AMB Express       Date:  2013-01-12       Impact factor: 3.298
  5 in total

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