PURPOSE: To demonstrate the role of N-cadherin (N-cad) in maintaining the progenitor status of primary human limbal epithelial cells in vitro. METHODS: Immunohistochemistry and immunoelectron microscopy against N-cad was performed in human limbal tissue. The expression of N-cad, cytokeratin (K) 12, and K14 was also observed in primary cultured limbal epithelial cell colonies in 3T3 feeder cells, which also express N-cad. Laser microdissection of individual colonies was performed to separate central cells from peripheral cells to compare secondary colony formation. Finally, an artificial small interfering RNA (siRNA) expression vector was used to downregulate N-cad in 3T3 cells (N-cad(low) 3T3) to observe changes in colony formation and cultivated epithelial sheet phenotype. RESULTS: N-cad was expressed in clusters of basal limbal epithelial cells. Limbal epithelial cell colonies cocultured with N-cad(+) 3T3 feeder cells showed N-cad expression along the edge of each colony. When individual colonies were divided into peripheral and central sections by laser microdissection, peripheral cells had a significantly higher secondary colony-forming efficiency than did central cells. Furthermore, colonies using N-cad(low) 3T3 cells were significantly smaller than mock-transfected cells. Then a duplex-feeder model with two layers of either 3T3 or N-cad(low) 3T3 cells was used to produce stratified epithelial sheets. Only N-cad(+) 3T3 cells produced epithelial sheets with basal K15/ suprabasal K12 expression observed in limbal tissue. CONCLUSIONS: N-cad plays a pivotal role in the maintenance of the progenitor phenotype in cultured limbal epithelial cells.
PURPOSE: To demonstrate the role of N-cadherin (N-cad) in maintaining the progenitor status of primary human limbal epithelial cells in vitro. METHODS: Immunohistochemistry and immunoelectron microscopy against N-cad was performed in human limbal tissue. The expression of N-cad, cytokeratin (K) 12, and K14 was also observed in primary cultured limbal epithelial cell colonies in 3T3 feeder cells, which also express N-cad. Laser microdissection of individual colonies was performed to separate central cells from peripheral cells to compare secondary colony formation. Finally, an artificial small interfering RNA (siRNA) expression vector was used to downregulate N-cad in 3T3 cells (N-cad(low) 3T3) to observe changes in colony formation and cultivated epithelial sheet phenotype. RESULTS:N-cad was expressed in clusters of basal limbal epithelial cells. Limbal epithelial cell colonies cocultured with N-cad(+) 3T3 feeder cells showed N-cad expression along the edge of each colony. When individual colonies were divided into peripheral and central sections by laser microdissection, peripheral cells had a significantly higher secondary colony-forming efficiency than did central cells. Furthermore, colonies using N-cad(low) 3T3 cells were significantly smaller than mock-transfected cells. Then a duplex-feeder model with two layers of either 3T3 or N-cad(low) 3T3 cells was used to produce stratified epithelial sheets. Only N-cad(+) 3T3 cells produced epithelial sheets with basal K15/ suprabasal K12 expression observed in limbal tissue. CONCLUSIONS:N-cad plays a pivotal role in the maintenance of the progenitor phenotype in cultured limbal epithelial cells.
Authors: Ana de la Mata; Teresa Nieto-Miguel; Marina López-Paniagua; Sara Galindo; María Rosa Aguilar; Luis García-Fernández; Sandra Gonzalo; Blanca Vázquez; Julio San Román; Rosa María Corrales; Margarita Calonge Journal: J Mater Sci Mater Med Date: 2013-07-28 Impact factor: 3.896
Authors: Hua Mei; Sheyla González; Martin N Nakatsu; Elfren Ray Baclagon; Vanda S Lopes; David S Williams; Sophie X Deng Journal: Tissue Eng Part C Methods Date: 2013-11-07 Impact factor: 3.056
Authors: Marc A Dziasko; Hannah E Armer; Hannah J Levis; Alex J Shortt; Stephen Tuft; Julie T Daniels Journal: PLoS One Date: 2014-04-08 Impact factor: 3.240
Authors: R Sartaj; C Zhang; P Wan; Z Pasha; V Guaiquil; A Liu; J Liu; Y Luo; E Fuchs; M I Rosenblatt Journal: Sci Rep Date: 2017-06-19 Impact factor: 4.379