We present a novel convergence of quantum-dot-mediated fluorescence resonance energy transfer (QD-FRET) and microfluidics, through which molecular interactions were precisely controlled and monitored using highly sensitive quantum-dot-mediated FRET. We demonstrate its potential in studying the kinetics of self-assembly of DNA polyplexes under laminar flow in real time with millisecond resolution. The integration of nanophotonics and microfluidics offers a powerful tool for elucidating the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.
We present a novel convergence of quantum-dot-mediated fluorescence resonance energy transfer (QD-FRET) and microfluidics, through which molecular interactions were precisely controlled and monitored using highly sensitive quantum-dot-mediated FRET. We demonstrate its potential in studying the kinetics of self-assembly of Dn class="Chemical">NA polyplexes under laminpan>ar flow inpan> real time with millisecond resolution. The inpan>tegration of nanpan>ophotonics anpan>d microfluidics offers a powerful tool for elucidatinpan>g the formation of polyelectrolyte polyplexes, which is expected to provide better control anpan>d synpan>thesis of uniform anpan>d customizable polyplexes for future nucleic acid-based therapeutics.