Jie Shen1, Ronghui Li, Gang Li. 1. Department of Neurosurgery, Qi Lu Hospital of Shandong University, 107 Wenhua Western Road, Jinan 250012, Shandong Province, PR China.
Abstract
BACKGROUND: It has been previously found that a constitutively activated signal transducer and activator of transcription 3 (STAT3) blocked by decoy-ODN led to glioma growth inhibition in vitro. The objectives of this study were to identify whether STAT3 decoy-ODN had the same effect or not in vivo. MATERIALS AND METHODS: Western blot was used to detect p-STAT3 in glioma and normal brain tissue. U251 cells were subcutaneously injected into nude mice and decoy-ODN was intratumorally administrated. TUNEL was used to exam the apoptosis cells in xenografts. Genes regulated by STAT3 were evaluated by RT-PCR and immunohistochemistry. RESULTS: Activated STAT3 was highly present in glioma but not normal brain tissues. STAT3 decoy-ODN could significantly suppress the growth of glioma by inhibiting proliferation and promoting apoptosis in xenografts. The target genes controlled by STAT3 were down-regulated at both transcription and translation levels. CONCLUSION: The present study suggested that decoy-ODN provide an effective therapeutic approach to treat glioma in vivo.
BACKGROUND: It has been previously found that a constitutively activated signal transducer and activator of transcription 3 (STAT3) blocked by decoy-ODN led to glioma growth inhibition in vitro. The objectives of this study were to identify whether STAT3 decoy-ODN had the same effect or not in vivo. MATERIALS AND METHODS: Western blot was used to detect p-STAT3 in glioma and normal brain tissue. U251 cells were subcutaneously injected into nude mice and decoy-ODN was intratumorally administrated. TUNEL was used to exam the apoptosis cells in xenografts. Genes regulated by STAT3 were evaluated by RT-PCR and immunohistochemistry. RESULTS: Activated STAT3 was highly present in glioma but not normal brain tissues. STAT3 decoy-ODN could significantly suppress the growth of glioma by inhibiting proliferation and promoting apoptosis in xenografts. The target genes controlled by STAT3 were down-regulated at both transcription and translation levels. CONCLUSION: The present study suggested that decoy-ODN provide an effective therapeutic approach to treat glioma in vivo.
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