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Literature DB >> 19411411

Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil.

Caroline Heath¹, Xiao Ping Hu, S Craig Cary, Donald Cowan.

Abstract

A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application.

Entities: Species

Mesh：

Substances：
DNA
Esterases

Year: 2009 PMID： 19411411 PMCID： PMC2704825 DOI： 10.1128/AEM.02597-08

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

23 in total

1. Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil.

Authors: Nathalie Wery; Ursula Gerike; Ajay Sharman; Julian B Chaudhuri; David W Hough; Michael J Danson
Journal: Appl Environ Microbiol Date: 2003-03 Impact factor: 4.792

2. Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability.

Authors: Ljudmila Kulakova; Andrey Galkin; Toru Nakayama; Tokuzo Nishino; Nobuyoshi Esaki
Journal: Biochim Biophys Acta Date: 2004-01-14

3. The alpha/beta hydrolase fold.

Authors: D L Ollis; E Cheah; M Cygler; B Dijkstra; F Frolow; S M Franken; M Harel; S J Remington; I Silman; J Schrag
Journal: Protein Eng Date: 1992-04

4. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.

Authors: R Eisenthal; A Cornish-Bowden
Journal: Biochem J Date: 1974-06 Impact factor: 3.857

5. Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass.

Authors: Don A Cowan; Nick J Russell; Adam Mamais; Devon M Sheppard
Journal: Extremophiles Date: 2002-07-30 Impact factor: 2.395

6. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A.

Authors: N Rashid; Y Shimada; S Ezaki; H Atomi; T Imanaka
Journal: Appl Environ Microbiol Date: 2001-09 Impact factor: 4.792

7. Bacterial diversity in three different Antarctic Cold Desert mineral soils.

Authors: Jacques J Smith; Lemese Ah Tow; William Stafford; Craig Cary; Donald A Cowan
Journal: Microb Ecol Date: 2006-04-05 Impact factor: 4.552

8. Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi.

Authors: Ying Xu; Georges Feller; Charles Gerday; Nicolas Glansdorff
Journal: J Bacteriol Date: 2003-04 Impact factor: 3.490

9. Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?

Authors: Ying Xu; Georges Feller; Charles Gerday; Nicolas Glansdorff
Journal: J Bacteriol Date: 2003-09 Impact factor: 3.490

10. A serine protease triad forms the catalytic centre of a triacylglycerol lipase.

Authors: L Brady; A M Brzozowski; Z S Derewenda; E Dodson; G Dodson; S Tolley; J P Turkenburg; L Christiansen; B Huge-Jensen; L Norskov
Journal: Nature Date: 1990-02-22 Impact factor: 49.962

30 in total

1. Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.

Authors: Kyong-Cheol Ko; Soon-Ok Rim; Yunjon Han; Bong Seok Shin; Geun-Joong Kim; Jong Hyun Choi; Jae Jun Song
Journal: J Ind Microbiol Biotechnol Date: 2012-01-24 Impact factor: 3.346

2. Novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase.

Authors: Mi-Hwa Lee; Ki-Hoon Oh; Chul-Hyung Kang; Ji-Hoon Kim; Tae-Kwang Oh; Choong-Min Ryu; Jung-Hoon Yoon
Journal: Appl Environ Microbiol Date: 2012-04-27 Impact factor: 4.792

Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil.

1. Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil.

2. Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability.

3. The alpha/beta hydrolase fold.

4. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.

5. Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass.

6. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A.

7. Bacterial diversity in three different Antarctic Cold Desert mineral soils.

8. Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi.

9. Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?

10. A serine protease triad forms the catalytic centre of a triacylglycerol lipase.

1. Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.

2. Novel metagenome-derived, cold-adapted alkaline phospholipase with superior lipase activity as an intermediate between phospholipase and lipase.

Review 3. Metagenomic analyses: past and future trends.

4. Size Does Matter: Application-driven Approaches for Soil Metagenomics.

5. Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome.

6. Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library.

7. A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

Review 8. New extremophilic lipases and esterases from metagenomics.

Review 9. Achievements and new knowledge unraveled by metagenomic approaches.

10. Probiotics and gastrointestinal disease: successes, problems and future prospects.