Lei Wang1, Dian-Ming Jiang. 1. Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, #1 Youyi Rd, Chongqing 400016, China. wlei 78@yahoo.com.cn
Abstract
AIM OF THE STUDY: The aim of this study was to investigate the protective effect of Buyang Huanwu Decoction, a traditional Chinese medicine formula, on spinal ischemia/reperfusion injury and explore the possible mechanism of the protective effect. MATERIALS AND METHODS: The spinal ischemia/reperfusion injury model was conducted in male Sprague-Dawley rats, and 40 g/kg Buyang Huanwu Decoction was administered by introgastric infusion. Motor function of hind limbs and apoptosis index were measured 72 h after reperfusion was started. The expression of thioredoxin and thioredoxin reductase was examined at 6h and at 24h after reperfusion. RESULTS: Motor function scores and apoptosis indices were significantly improved in the Buyang Huanwu Decoction group, as compared to the saline-infused control group. Spinal ischemia/reperfusion injury resulted in a decrease in the expression of thioredoxin, while Buyang Huanwu Decoction administration greatly elevated the expression of thioredoxin-1/thioredoxin-2 mRNA and thioredoxin reductase-1/thioredoxin reductase-2 mRNA. CONCLUSIONS: Our results suggest that administration of Buyang Huanwu Decoction may reduce spinal ischemia/reperfusion damage. This neuroprotective effect may be mediated, in part, by an increase in the transcription of thioredoxin.
AIM OF THE STUDY: The aim of this study was to investigate the protective effect of Buyang Huanwu Decoction, a traditional Chinese medicine formula, on spinal ischemia/reperfusion injury and explore the possible mechanism of the protective effect. MATERIALS AND METHODS: The spinal ischemia/reperfusion injury model was conducted in male Sprague-Dawley rats, and 40 g/kg Buyang Huanwu Decoction was administered by introgastric infusion. Motor function of hind limbs and apoptosis index were measured 72 h after reperfusion was started. The expression of thioredoxin and thioredoxin reductase was examined at 6h and at 24h after reperfusion. RESULTS: Motor function scores and apoptosis indices were significantly improved in the Buyang Huanwu Decoction group, as compared to the saline-infused control group. Spinal ischemia/reperfusion injury resulted in a decrease in the expression of thioredoxin, while Buyang Huanwu Decoction administration greatly elevated the expression of thioredoxin-1/thioredoxin-2 mRNA and thioredoxin reductase-1/thioredoxin reductase-2 mRNA. CONCLUSIONS: Our results suggest that administration of Buyang Huanwu Decoction may reduce spinal ischemia/reperfusion damage. This neuroprotective effect may be mediated, in part, by an increase in the transcription of thioredoxin.
Authors: Bing Chun Yan; Joon Ha Park; Ji Hyeon Ahn; Young Joo Lee; Tae Hun Lee; Choong Hyun Lee; Jun Hwi Cho; Myong Jo Kim; Tae Young Kim; Il-Jun Kang; Moo-Ho Won Journal: Neurochem Res Date: 2012-01-14 Impact factor: 3.996
Authors: Ying Chun Zhou; Bin Liu; Ying Jia Li; Lin Lin Jing; Ge Wen; Jing Tang; Xin Xu; Zhi Ping Lv; Xue Gang Sun Journal: Evid Based Complement Alternat Med Date: 2012-09-19 Impact factor: 2.629